PMID- 22024398
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 179
IP  - 1
DP  - 2012 Jan
TI  - Detection of enterovirus RNA in cerebrospinal fluid: comparison of two molecular 
      assays.
PG  - 104-7
LID - 10.1016/j.jviromet.2011.10.007 [doi]
AB  - Enterovirus (EV) and human parechovirus (HPeV) are a major cause of infection in 
      childhood. A rapid diagnostic test may improve the management of patients with EV 
      and HPeV infection. The aim of this study is to evaluate the performance of the 
      GeneXpert enterovirus assay (GXEA) for detection of EV RNA compared to a 
      user-developed reverse-transcriptase (RT) quantitative real-time PCR (qPCR) in 
      routine clinical practice. Also a RT-qPCR assay for detection of HPeV RNA in 
      different clinical samples was developed and evaluated. Cerebrospinal fluid (CSF) 
      from 232 patients suspected for meningitis was collected and tested for EV and 
      HPeV using RT-qPCR assays. In parallel an aliquot of the samples was tested using 
      the GXEA and viral culture. EV RNA was detected in 22 (19.0%) and 28 (24.1%) of 
      116 samples using the GXEA and RT-qPCR assay, respectively. EV was isolated from 
      10 of 116 (8.6%) samples by viral culture. GXEA had a sensitivity, specificity, 
      positive predictive value and negative predictive value of 82.1%, 100%, 100% and 
      96.2%, respectively. In this study, molecular assays were superior to viral 
      culture for detecting EV RNA in CSF. GXEA showed a high specificity but a lower 
      sensitivity for the detection of EV RNA compared to the RT-qPCR assay.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - de Crom, S C M
AU  - de Crom SC
AD  - Department of Pediatrics, St. Elisabeth Hospital, Post-office Box 90151, 5000 LC, 
      Tilburg, The Netherlands.
FAU - Obihara, C C
AU  - Obihara CC
FAU - van Loon, A M
AU  - van Loon AM
FAU - Argilagos-Alvarez, A A
AU  - Argilagos-Alvarez AA
FAU - Peeters, M F
AU  - Peeters MF
FAU - van Furth, A M
AU  - van Furth AM
FAU - Rossen, J W A
AU  - Rossen JW
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20111015
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cerebrospinal Fluid/*virology
MH  - Child
MH  - Child, Preschool
MH  - Enterovirus/genetics/*isolation & purification
MH  - Enterovirus Infections/*diagnosis/virology
MH  - Female
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Meningoencephalitis/*diagnosis/virology
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - Parechovirus/genetics/isolation & purification
MH  - Picornaviridae Infections/diagnosis/virology
MH  - RNA, Viral/genetics/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Virology/*methods
MH  - Young Adult
EDAT- 2011/10/26 06:00
MHDA- 2012/04/20 06:00
CRDT- 2011/10/26 06:00
PHST- 2011/05/26 00:00 [received]
PHST- 2011/09/22 00:00 [revised]
PHST- 2011/10/10 00:00 [accepted]
PHST- 2011/10/26 06:00 [entrez]
PHST- 2011/10/26 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - S0166-0934(11)00406-X [pii]
AID - 10.1016/j.jviromet.2011.10.007 [doi]
PST - ppublish
SO  - J Virol Methods. 2012 Jan;179(1):104-7. doi: 10.1016/j.jviromet.2011.10.007. Epub 
      2011 Oct 15.