PMID- 22024407
OWN - NLM
STAT- MEDLINE
DCOM- 20120522
LR  - 20161125
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 80
IP  - 2
DP  - 2012 Feb
TI  - Effect of surfactant on 5-aminolevulinic acid uptake and PpIX generation in human 
      cholangiocarcinoma cell.
PG  - 453-8
LID - 10.1016/j.ejpb.2011.10.008 [doi]
AB  - Photodynamic therapy (PDT) is a palliative therapy and has been used to cure 
      cholangiocarcinoma (CC), which has a poor prognosis and limited available 
      curative therapy. PDT was shown to improve the median survival time of 
      advanced-stage patients. Recently, 5-aminolevulinic acid (ALA) has been used as a 
      pro-photosensitizer, which can be transferred to intercellular protoporphyrin IX 
      (PpIX), which is a strong photosensitizer, via the heme pathway. The main 
      limitation of using ALA in PDT is the hydrophilic properties of ALA, which 
      results in low cellular uptake. In this study, non-ionic surfactants, pluronic 
      F68 (PF68) and Tween 80 (TW80), were used to address this limitation. The human 
      CC cell line, HuCC-T1, was cotreated with ALA and different concentrations of 
      surfactants for 4h. The effect of surfactants was evaluated by monitoring the 
      uptake of ALA, the fluorescence intensity of PpIX, and the cell survival rate 
      after suitable light irradiation. Cotreatment with the surfactant resulted in an 
      increased intracellular ALA level, PpIX formation, and phototoxicity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chung, Chung-Wook
AU  - Chung CW
AD  - Research Institute for Convergence of Biomedical Science and Technology, Pusan 
      National University Yangsan Hospital, Yangsan, Republic of Korea.
FAU - Kim, Cy Hyun
AU  - Kim CH
FAU - Choi, Kyung Ha
AU  - Choi KH
FAU - Yoo, Jin-Ju
AU  - Yoo JJ
FAU - Kim, Do Hyung
AU  - Kim DH
FAU - Chung, Kyu-Don
AU  - Chung KD
FAU - Jeong, Young-Il
AU  - Jeong YI
FAU - Kang, Dae Hwan
AU  - Kang DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111018
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Excipients)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Polysorbates)
RN  - 0 (Protoporphyrins)
RN  - 0 (Surface-Active Agents)
RN  - 106392-12-5 (Poloxamer)
RN  - 88755TAZ87 (Aminolevulinic Acid)
RN  - C2K325S808 (protoporphyrin IX)
SB  - IM
MH  - Aminolevulinic Acid/administration & dosage/pharmacokinetics/*pharmacology
MH  - Bile Duct Neoplasms/drug therapy/pathology
MH  - Bile Ducts, Intrahepatic/drug effects/pathology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cholangiocarcinoma/*drug therapy/pathology
MH  - Excipients/chemistry
MH  - Humans
MH  - Photochemotherapy/methods
MH  - Photosensitizing Agents/administration & dosage/pharmacokinetics/*pharmacology
MH  - Poloxamer/chemistry
MH  - Polysorbates/chemistry
MH  - Protoporphyrins/metabolism
MH  - Surface-Active Agents/*chemistry
EDAT- 2011/10/26 06:00
MHDA- 2012/05/23 06:00
CRDT- 2011/10/26 06:00
PHST- 2011/03/09 00:00 [received]
PHST- 2011/09/16 00:00 [revised]
PHST- 2011/10/07 00:00 [accepted]
PHST- 2011/10/26 06:00 [entrez]
PHST- 2011/10/26 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
AID - S0939-6411(11)00303-1 [pii]
AID - 10.1016/j.ejpb.2011.10.008 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2012 Feb;80(2):453-8. doi: 10.1016/j.ejpb.2011.10.008. Epub 
      2011 Oct 18.