PMID- 22024613
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20111025
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 135
IP  - 2
DP  - 2011
TI  - Human BRCA1-associated breast cancer: no increase in numerical chromosomal 
      instability compared to sporadic tumors.
PG  - 84-92
LID - 10.1159/000332005 [doi]
AB  - BRCA1 is a major gatekeeper of genomic stability. Acting in multiple central 
      processes like double-strand break repair, centrosome replication, and checkpoint 
      control, BRCA1 participates in maintaining genomic integrity and protects the 
      cell against genomic instability. Chromosomal instability (CIN) as part of 
      genomic instability is an inherent characteristic of most solid tumors and is 
      also involved in breast cancer development. In this study, we determined the 
      extent of CIN in 32 breast cancer tumors of women with a BRCA1 germline mutation 
      compared to 62 unselected breast cancers. We applied fluorescence in situ 
      hybridization (FISH) with centromere-specific probes for the chromosomes 1, 7, 8, 
      10, 17, and X and locus-specific probes for 3q27 (BCL6), 5p15.2 (D5S23), 5q31 
      (EGR1), 10q23.3 (PTEN), and 14q32 (IGH@) on formalin-fixed paraffin-embedded 
      tissue microarray sections. Our hypothesis of an increased level of CIN in 
      BRCA1-associated breast cancer could not be confirmed by this approach. 
      Surprisingly, we detected no significant difference in the extent of CIN in 
      BRCA1-mutated versus sporadic tumors. The only exception was the CIN value for 
      chromosome 1. Here, the extent of CIN was slightly higher in the group of 
      sporadic tumors.
CI  - Copyright (c) 2011 S. Karger AG, Basel.
FAU - Focken, T
AU  - Focken T
AD  - Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, 
      Germany.
FAU - Steinemann, D
AU  - Steinemann D
FAU - Skawran, B
AU  - Skawran B
FAU - Hofmann, W
AU  - Hofmann W
FAU - Ahrens, P
AU  - Ahrens P
FAU - Arnold, N
AU  - Arnold N
FAU - Kroll, P
AU  - Kroll P
FAU - Kreipe, H
AU  - Kreipe H
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Gadzicki, D
AU  - Gadzicki D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111024
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (BRCA1 Protein)
SB  - IM
MH  - BRCA1 Protein/*genetics
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosomal Instability
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 10/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Chromosomes, Human, X/genetics
MH  - Female
MH  - *Germ-Line Mutation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Tissue Array Analysis
EDAT- 2011/10/26 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/10/26 06:00
PHST- 2011/07/25 00:00 [accepted]
PHST- 2011/10/26 06:00 [entrez]
PHST- 2011/10/26 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 000332005 [pii]
AID - 10.1159/000332005 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2011;135(2):84-92. doi: 10.1159/000332005. Epub 2011 Oct 
      24.