PMID- 22025973
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 2093-3673 (Electronic)
IS  - 2093-3665 (Print)
IS  - 2093-3665 (Linking)
VI  - 44
IP  - 3
DP  - 2011 Sep
TI  - Modulation by the GABA(B) receptor siRNA of ethanol-mediated PKA-alpha, CaMKII, and 
      p-CREB intracellular signaling in prenatal rat hippocampal neurons.
PG  - 210-7
LID - 10.5115/acb.2011.44.3.210 [doi]
AB  - Fetal alcohol syndrome (FAS) is a developmental neuropathology resulting from in 
      utero exposure to ethanol; many of ethanol's effects are likely to be mediated by 
      the neurotransmitter gamma-aminobutyric acid (GABA). We studied modulation of the 
      neurotransmitter receptor GABA(B)R and its capacity for intracellular signal 
      transduction under conditions of ethanol treatment (ET) and RNA interference to 
      investigate a potential role for GABA signaling in FAS. ET increased GABA(B1)R 
      protein levels, but decreased protein kinase A-alpha (PKA-alpha), 
      calcium/calmodulin-dependent protein kinase II (CaMKII) and phosphorylation of 
      cAMP-response element binding protein (p-CREB), in cultured hippocampal neurons 
      harvested at gestation day 17.5. To elucidate GABA(B1)R response to ethanol, we 
      observed the effects of a GABA(B)R agonist and antagonist in pharmacotherapy for 
      ethanol abuse. Baclofen increased GABA(B)R, CaMKII and p-CREB levels, whereas 
      phaclofen decreased GABA(B)R, CaMKII and p-CREB levels except PKA-alpha. Furthermore, 
      when GABA(B1)R was knocked down by siRNA treatment, CaMKII and p-CREB levels were 
      reduced upon ET. We speculate that stimulation of GABA(B1)R activity by ET can 
      modulate CaMKII and p-CREB signaling to detrimental effect on fetal brain 
      development.
FAU - Lee, Hae Young
AU  - Lee HY
AD  - Department of Biology, College of Natural Sciences (RINS), Gyeongsang National 
      University, Jinju, Korea.
FAU - Yang, Byoung-Chul
AU  - Yang BC
FAU - Lee, Eun-Shil
AU  - Lee ES
FAU - Chung, Jong Ii
AU  - Chung JI
FAU - Koh, Phil Ok
AU  - Koh PO
FAU - Park, Moon Seok
AU  - Park MS
FAU - Kim, Myeong Ok
AU  - Kim MO
LA  - eng
PT  - Journal Article
DEP - 20110929
PL  - Korea (South)
TA  - Anat Cell Biol
JT  - Anatomy & cell biology
JID - 101531987
PMC - PMC3195825
OTO - NOTNLM
OT  - Ethanol
OT  - GABAB receptor
OT  - Hippocampus
OT  - p-CREB
OT  - siRNA
EDAT- 2011/10/26 06:00
MHDA- 2011/10/26 06:01
PMCR- 2011/09/01
CRDT- 2011/10/26 06:00
PHST- 2011/04/20 00:00 [received]
PHST- 2011/06/22 00:00 [revised]
PHST- 2011/06/30 00:00 [accepted]
PHST- 2011/10/26 06:00 [entrez]
PHST- 2011/10/26 06:00 [pubmed]
PHST- 2011/10/26 06:01 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - 10.5115/acb.2011.44.3.210 [doi]
PST - ppublish
SO  - Anat Cell Biol. 2011 Sep;44(3):210-7. doi: 10.5115/acb.2011.44.3.210. Epub 2011 
      Sep 29.