PMID- 22029418
OWN - NLM
STAT- MEDLINE
DCOM- 20120403
LR  - 20211203
IS  - 2152-4998 (Electronic)
IS  - 2152-4971 (Linking)
VI  - 13
IP  - 6
DP  - 2011 Dec
TI  - Histone deacetylase inhibitor significantly improved the cloning efficiency of 
      porcine somatic cell nuclear transfer embryos.
PG  - 513-20
LID - 10.1089/cell.2011.0032 [doi]
AB  - Valproic acid (VPA), a histone deacetylase inbibitor, has been shown to generate 
      inducible pluripotent stem (iPS) cells from mouse and human fibroblasts with a 
      significant higher efficiency. Because successful cloning by somatic cell nuclear 
      transfer (SCNT) undergoes a full reprogramming process in which the epigenetic 
      state of a differentiated donor nuclear is converted into an embryonic totipotent 
      state, we speculated that VPA would be useful in promoting cloning efficiency. 
      Therefore, in the present study, we examined whether VPA can promote the 
      developmental competence of SCNT embryos by improving the reprogramming state of 
      donor nucleus. Here we report that 1 mM VPA for 14 to 16 h following activation 
      significantly increased the rate of blastocyst formation of porcine SCNT embryos 
      constructed from Landrace fetal fibroblast cells compared to the control (31.8 
      vs. 11.4%). However, we found that the acetylation level of Histone H3 lysine 14 
      and Histone H4 lysine 5 and expression level of Oct4, Sox2, and Klf4 was not 
      significantly changed between VPA-treated and -untreated groups at the blastocyst 
      stage. The SCNT embryos were transferred to 38 surrogates, and the cloning 
      efficiency in the treated group was significantly improved compared with the 
      control group. Taken together, we have demonstrated that VPA can improve both in 
      vitro and in vivo development competence of porcine SCNT embryos.
FAU - Huang, Yongye
AU  - Huang Y
AD  - Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal 
      Science and Veterinary Medicine, Jilin University, Changchun, People's Republic 
      of China.
FAU - Tang, Xiaochun
AU  - Tang X
FAU - Xie, Wanhua
AU  - Xie W
FAU - Zhou, Yan
AU  - Zhou Y
FAU - Li, Dong
AU  - Li D
FAU - Yao, Chaogang
AU  - Yao C
FAU - Zhou, Yang
AU  - Zhou Y
FAU - Zhu, Jianguo
AU  - Zhu J
FAU - Lai, Liangxue
AU  - Lai L
FAU - Ouyang, Hongsheng
AU  - Ouyang H
FAU - Pang, Daxin
AU  - Pang D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111026
PL  - United States
TA  - Cell Reprogram
JT  - Cellular reprogramming
JID - 101528176
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Histones)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Transcription Factors)
RN  - 614OI1Z5WI (Valproic Acid)
SB  - IM
MH  - Acetylation/drug effects
MH  - Animals
MH  - Blastocyst/cytology/*metabolism
MH  - Cell Dedifferentiation/*drug effects
MH  - Cloning, Organism/*methods
MH  - Epigenesis, Genetic/drug effects
MH  - Female
MH  - Histone Deacetylase Inhibitors/*pharmacology
MH  - Histones/metabolism
MH  - Kruppel-Like Factor 4
MH  - *Nuclear Transfer Techniques
MH  - Swine
MH  - Transcription Factors/metabolism
MH  - Valproic Acid/*pharmacology
EDAT- 2011/10/28 06:00
MHDA- 2012/04/04 06:00
CRDT- 2011/10/28 06:00
PHST- 2011/10/28 06:00 [entrez]
PHST- 2011/10/28 06:00 [pubmed]
PHST- 2012/04/04 06:00 [medline]
AID - 10.1089/cell.2011.0032 [doi]
PST - ppublish
SO  - Cell Reprogram. 2011 Dec;13(6):513-20. doi: 10.1089/cell.2011.0032. Epub 2011 Oct 
      26.