PMID- 22029600
OWN - NLM
STAT- MEDLINE
DCOM- 20120307
LR  - 20111124
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 63
IP  - 3
DP  - 2011 Dec
TI  - Mouse model for hemolytic uremic syndrome induced by outer membrane vesicles of 
      Escherichia coli O157:H7.
PG  - 427-34
LID - 10.1111/j.1574-695X.2011.00869.x [doi]
AB  - Hemolytic uremic syndrome (HUS) is characterized by acute renal failure in 
      children and is typically complicated with thrombocytopenia and hemolytic anemia. 
      Although mouse models of HUS have been evaluated using Shiga toxin (STx) combined 
      with or without lipopolysaccharide (LPS), no HUS model has been tested using 
      purified outer membrane vesicles (OMVs) from STx-producing Escherichia coli 
      (STEC) O157:H7. Accordingly, we investigated whether OMVs of STEC O157:H7 
      conveying STx2 and LPS can cause HUS-like symptoms in mice inoculated 
      intraperitoneally. Three types of OMVs differing in LPS acylation status and STx2 
      amount were used to compare their ability to induce HUS-like symptoms. Native 
      OMVs (nOMV) with fully hexa-acylated LPS caused HUS-like symptoms at 72-96 h 
      after dually divided injections of 1 mug nOMV per animal. However, elevated doses 
      of modified OMVs (mOMV) carrying mainly penta-acylated LPS were required to 
      induce similar HUS signs. Moreover, mitomycin-C-induced OMVs (mcOMV) that were 
      enriched with STx2 induced HUS-like symptoms similar to those of nOMV at the same 
      dose. These results suggest that the OMVs of STEC O157:H7 potentiated with STx2 
      and fully hexa-acylated LPS can be utilized for inducing HUS-like symptoms in 
      mice and could be the causative material involved in the development of HUS.
CI  - (c) 2011 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Kim, Sang-Hyun
AU  - Kim SH
AD  - The National Primate Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Ochang, Cheongwon, Chungbuk, Korea.
FAU - Lee, Yong-Hoon
AU  - Lee YH
FAU - Lee, Sang-Ho
AU  - Lee SH
FAU - Lee, Sang-Rae
AU  - Lee SR
FAU - Huh, Jae-Won
AU  - Huh JW
FAU - Kim, Sun-Uk
AU  - Kim SU
FAU - Chang, Kyu-Tae
AU  - Chang KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111004
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Shiga Toxin 2)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Escherichia coli O157/*pathogenicity
MH  - Exosomes/*metabolism
MH  - Hemolytic-Uremic Syndrome/*chemically induced/*pathology
MH  - Lipopolysaccharides/administration & dosage/*toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Shiga Toxin 2/administration & dosage/*toxicity
EDAT- 2011/10/28 06:00
MHDA- 2012/03/08 06:00
CRDT- 2011/10/28 06:00
PHST- 2011/05/05 00:00 [received]
PHST- 2011/08/08 00:00 [revised]
PHST- 2011/09/02 00:00 [accepted]
PHST- 2011/10/28 06:00 [entrez]
PHST- 2011/10/28 06:00 [pubmed]
PHST- 2012/03/08 06:00 [medline]
AID - 10.1111/j.1574-695X.2011.00869.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2011 Dec;63(3):427-34. doi: 
      10.1111/j.1574-695X.2011.00869.x. Epub 2011 Oct 4.