PMID- 22029937
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20181201
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 421
IP  - 2
DP  - 2011 Dec 20
TI  - Non-genotype-specific role of the hepatitis C virus 5' untranslated region in 
      virus production and in inhibition by interferon.
PG  - 222-34
LID - 10.1016/j.virol.2011.10.002 [doi]
AB  - The 5' untranslated region (5'UTR) of hepatitis C virus (HCV) is structured into 
      four domains (I-IV) with numerous genotype-specific nucleotides. It is unknown 
      whether the polymorphisms confer genotype-specific functions to the 5'UTR. Using 
      viable JFH1-based Core-NS2 recombinants, we developed and characterized HCV 
      genotypes 1-7 recombinants with highly diverse 5'UTRs (genotypes 1a and 3a), 2a 
      recombinants (J6/JFH1) with 5'UTR of genotypes 1-6 or with heterotypic chimeric 
      (1a/3a and 3a/1a) 5'UTR domains I, II or III, and 1a recombinants with 5'UTR 
      domain I of genotypes 1-6. All were fully functional in Huh7.5 cells; therefore, 
      the 5'UTR apparently functions in a non-genotype-specific manner in HCV 
      production in vitro. However, adenine at the 5'-terminus was required. We 
      demonstrated that J6/JFH1 with 5'UTR of genotypes 1-6 responded similarly to 
      interferon-alpha2b. This study provides novel insight into the role of the 5'UTR in 
      the HCV life cycle and facilitates HCV basic research and testing of 
      5'UTR-targeting antivirals.
CI  - Copyright A(c) 2011 Elsevier Inc. All rights reserved.
FAU - Li, Yi-Ping
AU  - Li YP
AD  - Copenhagen Hepatitis C Program, Department of Infectious Diseases and Clinical 
      Research Centre, Copenhagen University Hospital, Hvidovre, Denmark.
FAU - Ramirez, Santseharay
AU  - Ramirez S
FAU - Gottwein, Judith M
AU  - Gottwein JM
FAU - Bukh, Jens
AU  - Bukh J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111024
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Interferon alpha-2)
RN  - 0 (Interferon-alpha)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Base Sequence
MH  - Cell Line
MH  - Genotype
MH  - Hepacivirus/drug effects/*genetics/*physiology
MH  - Humans
MH  - Interferon alpha-2
MH  - Interferon-alpha/*pharmacology
MH  - RNA, Viral/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Sequence Analysis, DNA
MH  - Viral Core Proteins/genetics
MH  - Virus Replication/*drug effects/*genetics
EDAT- 2011/10/28 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/10/28 06:00
PHST- 2011/05/27 00:00 [received]
PHST- 2011/06/15 00:00 [revised]
PHST- 2011/10/03 00:00 [accepted]
PHST- 2011/10/28 06:00 [entrez]
PHST- 2011/10/28 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
AID - S0042-6822(11)00470-3 [pii]
AID - 10.1016/j.virol.2011.10.002 [doi]
PST - ppublish
SO  - Virology. 2011 Dec 20;421(2):222-34. doi: 10.1016/j.virol.2011.10.002. Epub 2011 
      Oct 24.