PMID- 22031309
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20111027
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 136
IP  - 5
DP  - 2011 Nov
TI  - Detection of genomic abnormalities in multiple myeloma: the application of FISH 
      analysis in combination with various plasma cell enrichment techniques.
PG  - 712-20
LID - 10.1309/AJCPF7NFLW8UAJEP [doi]
AB  - Multiple myeloma (MM) is a hematopoietic neoplasm characterized by malignant 
      plasma cells (PCs) that accumulate in the bone marrow. A number of different 
      genomic abnormalities are associated with MM; however, detection of these by 
      fluorescence in situ hybridization (FISH) can be limited by the percentage of PCs 
      in the specimen. In this study, we tested 20 bone marrow specimens with known MM 
      and a low concentration of monoclonal PCs for the presence of genomic 
      abnormalities using FISH in combination with various PC enrichment techniques: 
      magnetic cell sorting, targeted manual scoring, and automated image analysis. In 
      addition, flow cytometric cell sorting of PCs in combination with FISH analysis 
      was also tested for minimal residual disease applications. Different parameters 
      were evaluated when assessing the detection efficiency of each approach. FISH 
      results are highly dependent on the chosen enrichment method. We describe the 
      evaluation of different techniques applicable for various laboratory settings and 
      specimen parameters.
FAU - Hartmann, Luise
AU  - Hartmann L
AD  - Department of Molecular Analysis, HematoLogics, 3161 Elliott Avenue, Seattle, WA 
      98121, USA.
FAU - Biggerstaff, Julie Sanford
AU  - Biggerstaff JS
FAU - Chapman, Douglas B
AU  - Chapman DB
FAU - Scott, Janice M
AU  - Scott JM
FAU - Johnson, Krystal R
AU  - Johnson KR
FAU - Ghirardelli, Keely M
AU  - Ghirardelli KM
FAU - Fritschle, Wayne K
AU  - Fritschle WK
FAU - Martinez, Dolores L
AU  - Martinez DL
FAU - Bennington, Richard K
AU  - Bennington RK
FAU - de Baca, Monica E
AU  - de Baca ME
FAU - Wells, Denise A
AU  - Wells DA
FAU - Loken, Michael R
AU  - Loken MR
FAU - Zehentner, Barbara K
AU  - Zehentner BK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Bone Marrow/pathology
MH  - Chromosome Aberrations
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Multiple Myeloma/diagnosis/*genetics
MH  - Plasma Cells/*pathology
EDAT- 2011/10/28 06:00
MHDA- 2011/12/16 06:00
CRDT- 2011/10/28 06:00
PHST- 2011/10/28 06:00 [entrez]
PHST- 2011/10/28 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
AID - 136/5/712 [pii]
AID - 10.1309/AJCPF7NFLW8UAJEP [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Nov;136(5):712-20. doi: 10.1309/AJCPF7NFLW8UAJEP.