PMID- 22031944
OWN - NLM
STAT- MEDLINE
DCOM- 20120214
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 1
DP  - 2012 Jan
TI  - Multiple viral ligands naturally presented by different class I molecules in 
      transporter antigen processing-deficient vaccinia virus-infected cells.
PG  - 527-41
LID - 10.1128/JVI.05737-11 [doi]
AB  - The transporter associated with antigen processing (TAP) delivers the viral 
      proteolytic products generated by the proteasome in the cytosol to the 
      endoplasmic reticulum lumen that are subsequently recognized by cytotoxic T 
      lymphocytes (CTLs). However, several viral epitopes have been identified in 
      TAP-deficient models. Using mass spectrometry to analyze complex human leukocyte 
      antigen (HLA)-bound peptide pools isolated from large numbers of TAP-deficient 
      vaccinia virus-infected cells, we identified 11 ligands naturally presented by 
      four different HLA-A, HLA-B, and HLA-C class I molecules. Two of these ligands 
      were presented by two different HLA class I alleles, and, as a result, 13 
      different HLA-peptide complexes were formed simultaneously in the same vaccinia 
      virus-infected cells. In addition to the high-affinity ligands, one low-affinity 
      peptide restricted by each of the HLA-A, HLA-B, and HLA-C class I molecules was 
      identified. Both high- and low-affinity ligands generated long-term memory CTL 
      responses to vaccinia virus in an HLA-A2-transgenic mouse model. The processing 
      and presentation of two vaccinia virus-encoded HLA-A2-restricted antigens took 
      place via proteasomal and nonproteasomal pathways, which were blocked in infected 
      cells with chemical inhibitors specific for different subsets of 
      metalloproteinases. These data have implications for the study of the 
      effectiveness of early empirical vaccination with cowpox virus against smallpox 
      disease.
FAU - Lorente, Elena
AU  - Lorente E
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Infantes, Susana
AU  - Infantes S
FAU - Barnea, Eilon
AU  - Barnea E
FAU - Beer, Ilan
AU  - Beer I
FAU - Garcia, Ruth
AU  - Garcia R
FAU - Lasala, Fatima
AU  - Lasala F
FAU - Jimenez, Mercedes
AU  - Jimenez M
FAU - Vilches, Carlos
AU  - Vilches C
FAU - Lemonnier, Francois A
AU  - Lemonnier FA
FAU - Admon, Arie
AU  - Admon A
FAU - Lopez, Daniel
AU  - Lopez D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111026
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Ligands)
RN  - 0 (transporter associated with antigen processing (TAP))
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/*immunology
MH  - Animals
MH  - Antigen Presentation
MH  - Antigen-Presenting Cells/immunology/virology
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Cell Line
MH  - Histocompatibility Antigens Class I/genetics/*immunology
MH  - Humans
MH  - Ligands
MH  - Mice
MH  - Mice, Knockout
MH  - Vaccinia/genetics/*immunology/virology
MH  - Vaccinia virus/genetics/*immunology
PMC - PMC3255926
EDAT- 2011/10/28 06:00
MHDA- 2012/02/15 06:00
PMCR- 2012/07/01
CRDT- 2011/10/28 06:00
PHST- 2011/10/28 06:00 [entrez]
PHST- 2011/10/28 06:00 [pubmed]
PHST- 2012/02/15 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - JVI.05737-11 [pii]
AID - 5737-11 [pii]
AID - 10.1128/JVI.05737-11 [doi]
PST - ppublish
SO  - J Virol. 2012 Jan;86(1):527-41. doi: 10.1128/JVI.05737-11. Epub 2011 Oct 26.