PMID- 22032158
OWN - NLM
STAT- MEDLINE
DCOM- 20120802
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 6
DP  - 2011 Jun
TI  - [An ultra performance liquid chromatography-time-of-flight-mass spectrometric 
      method for fast analysis of ginsenosides in Panax ginseng root].
PG  - 488-94
AB  - A method for fast analysis of ginsenosides in Panax ginseng roots was developed 
      using ultra performance liquid chromatography-time-of-flight-mass spectrometry 
      (UPLC-TOF-MS). The column used was HSS T3 (100 mm x 2.1 mm, 1.8 microm). The 
      mobile phase consisted of 15 mmol/L ammonium formate and acetonitrile, eluted 
      with the gradient program. The separations of 9 ginsenoside standards and ginseng 
      root extracts were achieved. Based on the MS/ MS fragments and accurate masses of 
      the target compounds and with combination of the MS/ MS fragments of the 9 
      ginsenoside standards, 27 ginsenosides were identified from the extracts of the 
      ginseng roots. The validation of the analytical method was thoroughly 
      investigated with 9 ginsenoside standards. It was found that 9 ginsenosides had a 
      better linearity in 0.04 - 9.00 mg/L. The recoveries at the three spiked levels 
      (low, medium and high) were 90% - 100%, 98% - 104% and 96% - 103%, respectively. 
      The relative standard deviations (RSDs) of the peak area ratio of 9 ginsenoside 
      standards to internal standard at the medium spiked level were not more than 
      11.3%, which were satisfactory for profiling analysis of herb extracts. This 
      method is characterized by its high resolution, rapidness, simplicity and 
      reliability, and has been successfully applied to the evaluation of the 
      differentiation between 2- and 6-year-old ginseng roots. It can be expected that 
      this method is also useful for the fast determination of the ginsenosides in 
      other ginseng related samples.
FAU - Hu, Chunxiu
AU  - Hu C
AD  - Dalian Institute of Chemical Physics, CAS Key Laboratory of Separation Science 
      for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China.
FAU - Kong, Hongwei
AU  - Kong H
FAU - Zhu, Chao
AU  - Zhu C
FAU - Wei, Heng
AU  - Wei H
FAU - Hankemeier, Thomas
AU  - Hankemeier T
FAU - van der Greef, Jan
AU  - van der Greef J
FAU - Wang, Mei
AU  - Wang M
FAU - Xu, Guowang
AU  - Xu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Ginsenosides)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Ginsenosides/*analysis
MH  - Mass Spectrometry/*methods
MH  - Panax/*chemistry
MH  - Plant Roots/chemistry
EDAT- 2011/10/29 06:00
MHDA- 2012/08/03 06:00
CRDT- 2011/10/29 06:00
PHST- 2011/10/29 06:00 [entrez]
PHST- 2011/10/29 06:00 [pubmed]
PHST- 2012/08/03 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.00488 [doi]
PST - ppublish
SO  - Se Pu. 2011 Jun;29(6):488-94. doi: 10.3724/sp.j.1123.2011.00488.