PMID- 22033289
OWN - NLM
STAT- MEDLINE
DCOM- 20120619
LR  - 20120207
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 421
IP  - 2
DP  - 2012 Feb 15
TI  - Microwave-mediated enzyme-linked immunosorbent assay procedure.
PG  - 764-6
LID - 10.1016/j.ab.2011.09.029 [doi]
AB  - Here we demonstrate a novel microwave-mediated enzyme-linked immunosorbent assay 
      (MELISA) method that has dramatically reduced the enzyme-linked immunosorbent 
      assay (ELISA) timing to less than 5 min with a result comparable to that obtained 
      by 18-h conventional ELISA. Efficacy of the MELISA procedure is demonstrated by 
      detecting human immunoglobulin G (IgG), rabbit IgG, human immunoglobulin E (IgE), 
      human interleuken 1beta (IL-1beta), Entamoeba histolytica antibody, and Aspergillus 
      fumigatus antibody. MELISA could be an excellent substitute for time-consuming 
      conventional ELISA for rapid diagnosis of diseases in cases of medical urgency, 
      outbreak of infectious diseases, and screening of samples in blood banks or 
      emigration counters.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Nahar, Pradip
AU  - Nahar P
AD  - Institute of Genomics and Integrative Biology (CSIR), Delhi 110007, India. 
      pnahar@igib.res.in
FAU - Bora, Utpal
AU  - Bora U
FAU - Sharma, Gainda L
AU  - Sharma GL
FAU - Kannoujia, Dileep Kumar
AU  - Kannoujia DK
LA  - eng
PT  - Journal Article
DEP - 20111006
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Immunoglobulins/analysis
MH  - *Microwaves
MH  - Rabbits
EDAT- 2011/10/29 06:00
MHDA- 2012/06/20 06:00
CRDT- 2011/10/29 06:00
PHST- 2011/05/24 00:00 [received]
PHST- 2011/09/23 00:00 [revised]
PHST- 2011/09/29 00:00 [accepted]
PHST- 2011/10/29 06:00 [entrez]
PHST- 2011/10/29 06:00 [pubmed]
PHST- 2012/06/20 06:00 [medline]
AID - S0003-2697(11)00638-5 [pii]
AID - 10.1016/j.ab.2011.09.029 [doi]
PST - ppublish
SO  - Anal Biochem. 2012 Feb 15;421(2):764-6. doi: 10.1016/j.ab.2011.09.029. Epub 2011 
      Oct 6.