PMID- 22035308
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR  - 20111031
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 28
IP  - 11
DP  - 2011 Nov
TI  - Differentiation of steroid-producing cells and folliculogenesis in the developing 
      ovary of the Nile tilapia Oreochromis niloticus.
PG  - 845-52
LID - 10.2108/zsj.28.845 [doi]
AB  - Differentiation and development of steroid-producing cells (SPCs) and 
      folliculogenesis during ovarian differentiation in the Nile tilapia Oreochromis 
      niloticus were immunohistochemically and ultrastructurally examined. Clusters of 
      immunopositive cells (IPCs) against antibodies (ABs) of cholesterol side-chain 
      cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase (3betaHSD), and 
      cytochrome P450aromatase (P450arom) only appeared in the area near blood vessels 
      in the fish ovaries at 50-60 days after hatching (dah). Ultrastructural results 
      showed that differentiation and development of SPCs from undifferentiated to 
      maturation occurred in the area near blood vessels, indicating that it would be 
      the original site of SPCs. At 70-80 dah, IPC clusters invaded the interstices 
      among oocytes at the perinucleolar stage from the area near the blood vessels. 
      IPCs increased in number in the interstices among the previtellogenic oocytes, 
      and some clusters began to enclose the outer thecal layer of the previtellogenic 
      oocytes at 90 dah. The process of folliculogenesis was ultrastructurally 
      observed. SPCs enclosed by fibroblastic cells invaded the interstitial areas 
      among oocytes and some reached the surfaces of oocytes. The upper portions of 
      these elongations opened and began to enclose the outer surfaces of developed 
      oocytes to become thecal layer. Later, newly migrated SPCs reach the thecal layer 
      to become thecal cells. These results indicate that steroid-producing thecal 
      cells originate from the SPCs in the area near blood vessels. After thecal layer 
      formation, an immunopositive reaction against P450arom AB, but not against 
      P450scc or 3beta-HSD ABs, appeared first in the granulosa cells enclosing the 
      vitellogenic oocytes at 100 dah. At this time, estrogen production in serum 
      levels rapidly increased. Thus, folliculogenesis could be essential for active 
      production of estrogen in the ovary.
FAU - Ruksana, Sabina
AU  - Ruksana S
AD  - Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 
      Sesoko 3422, Motobu, Okinawa 905-0227, Japan.
FAU - Alam, Mohammad Ashraful
AU  - Alam MA
FAU - Kobayashi, Yasuhisa
AU  - Kobayashi Y
FAU - Nakamura, Masaru
AU  - Nakamura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Steroids)
SB  - IM
MH  - Animals
MH  - Cichlids/*growth & development/*metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Ovary/*growth & development/*metabolism/ultrastructure
MH  - Steroids/*metabolism
EDAT- 2011/11/01 06:00
MHDA- 2012/03/07 06:00
CRDT- 2011/11/01 06:00
PHST- 2011/11/01 06:00 [entrez]
PHST- 2011/11/01 06:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
AID - 10.2108/zsj.28.845 [doi]
PST - ppublish
SO  - Zoolog Sci. 2011 Nov;28(11):845-52. doi: 10.2108/zsj.28.845.