PMID- 2203811
OWN - NLM
STAT- MEDLINE
DCOM- 19901011
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 8
DP  - 1990 Aug
TI  - Detection of specific immunoglobulin E in patients with toxoplasmosis.
PG  - 1739-43
AB  - An immunocapture assay was developed to detect Toxoplasma gondii-specific 
      immunoglobulin E (IgE) in sera from adults with acute acquired infection or 
      reactivation and from babies with congenital toxoplasmosis. The components of 
      this assay were monoclonal antibody to human IgE, samples from patients, and T. 
      gondii tachyzoites treated with Formalin. When T. gondii-specific IgE antibodies 
      were present, visually detectable agglutination occurred. Sera, umbilical cord 
      blood, fetal blood, cerebrospinal fluid, and amniotic fluid were tested by this 
      method. Specific IgE antibodies were detected in sera from 25 (86%) of 29 adults 
      who developed specific IgG antibody during pregnancy or had specific IgA and IgM 
      antibodies. Specific IgE was present early during infection, at the time that IgM 
      antibodies were present, and slightly preceding the presence of specific IgA 
      antibodies. In 23 patients tested serially, IgE antibodies never persisted for 
      longer than 4 months. No nonspecific anti-T. gondii IgE was detected in sera from 
      uninfected individuals. Maternal IgE antibodies did not cross the placenta. In 
      sera of patients with congenital toxoplasmosis, specific IgE antibodies were 
      found at birth, during the first year of life, and during immunologic 
      recrudescence following discontinuation of pyrimethamine-sulfonamide therapy. The 
      IgE immunocapture assay is simple to perform. It is especially useful for 
      determining when T. gondii was acquired by recently infected pregnant women.
FAU - Pinon, J M
AU  - Pinon JM
AD  - Laboratoire de Parasitologie, CHRU, Hopital Maison Blanche, Reims, France.
FAU - Toubas, D
AU  - Toubas D
FAU - Marx, C
AU  - Marx C
FAU - Mougeot, G
AU  - Mougeot G
FAU - Bonnin, A
AU  - Bonnin A
FAU - Bonhomme, A
AU  - Bonhomme A
FAU - Villaume, M
AU  - Villaume M
FAU - Foudrinier, F
AU  - Foudrinier F
FAU - Lepan, H
AU  - Lepan H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin M)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/complications/*immunology
MH  - Adult
MH  - Animals
MH  - Antibodies, Protozoan/*immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin M/immunology
MH  - Infant, Newborn
MH  - Infectious Mononucleosis/complications/immunology
MH  - Pregnancy
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Toxoplasma/*immunology
MH  - Toxoplasmosis, Congenital/*immunology
MH  - Uterine Cervical Diseases/complications/immunology
PMC - PMC268039
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
PMCR- 1990/08/01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
PHST- 1990/08/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.8.1739-1743.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 Aug;28(8):1739-43. doi: 10.1128/jcm.28.8.1739-1743.1990.