PMID- 22040047
OWN - NLM
STAT- MEDLINE
DCOM- 20120530
LR  - 20181201
IS  - 1348-0421 (Electronic)
IS  - 0385-5600 (Linking)
VI  - 56
IP  - 1
DP  - 2012 Jan
TI  - Anti-inflammatory properties of intestinal Bifidobacterium strains isolated from 
      healthy infants.
PG  - 27-39
LID - 10.1111/j.1348-0421.2011.00398.x [doi]
AB  - Certain Bifidobacterium strains have been shown to inhibit inflammatory responses 
      in intestinal epithelial cells. However, the precise mechanisms of these effects, 
      including the chemical nature of the active compounds, remain to be elucidated. 
      Here partial characterization of the anti-inflammatory properties of 
      Bifidobacterium strains isolated from feces of healthy infants is reported. It 
      was found that conditioned media (CM) of all strains studied are capable of 
      attenuating tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharide- (LPS) induced 
      inflammatory responses in the HT-29 cell line. In contrast, neither killed 
      bifidobacterial cells, nor cell-free extracts showed such activities. Further 
      investigations resulted in attribution of this activity to heat-stable, 
      non-lipophilic compound(s) resistant to protease and nuclease treatments and of 
      molecular weight less than 3 kDa. The anti-inflammatory effects were dose- and 
      time-dependent and associated with inhibition of IkappaB phosphorylation and nuclear 
      factor-kappa light chain enhancer of activated B cells (NF-kappaB)-dependent promoter 
      activation. The combined treatments of cells with CMs and either LPS or TNF-alpha, 
      but not with CMs alone, resulted in upregulation of transforming growth 
      factor-beta1, IkappaBzeta, and p21(CIP) mRNAs. Our data suggest certain 
      species-specificities of the anti-inflammatory properties of bifidobacteria. This 
      observation should prompt additional validation studies using larger set of 
      strains and employing the tools of comparative genomics.
CI  - (c) 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
FAU - Khokhlova, Ekaterina V
AU  - Khokhlova EV
AD  - Department of Microbiology and Virology, Russian State Medical University, 
      Moscow, Russia.
FAU - Smeianov, Vladimir V
AU  - Smeianov VV
FAU - Efimov, Boris A
AU  - Efimov BA
FAU - Kafarskaia, Lyudmila I
AU  - Kafarskaia LI
FAU - Pavlova, Svetlana I
AU  - Pavlova SI
FAU - Shkoporov, Andrei N
AU  - Shkoporov AN
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
SB  - IM
MH  - Apoptosis
MH  - Bifidobacterium/genetics/*immunology/*isolation & purification
MH  - Culture Media, Conditioned/pharmacology
MH  - Dose-Response Relationship, Immunologic
MH  - Escherichia coli/genetics/metabolism
MH  - Feces/microbiology
MH  - Gene Expression Regulation, Bacterial
MH  - HT29 Cells
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Infant
MH  - Inflammation/chemically induced/*microbiology
MH  - Interleukin-8/metabolism
MH  - Intestines/*microbiology
MH  - Lipopolysaccharides/adverse effects
MH  - Molecular Weight
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/antagonists & inhibitors/immunology/metabolism
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Species Specificity
MH  - Time Factors
MH  - Transcriptional Activation
MH  - Transfection
MH  - Transforming Growth Factor alpha/metabolism
MH  - Tumor Necrosis Factor-alpha/immunology/pharmacology
EDAT- 2011/11/02 06:00
MHDA- 2012/05/31 06:00
CRDT- 2011/11/02 06:00
PHST- 2011/11/02 06:00 [entrez]
PHST- 2011/11/02 06:00 [pubmed]
PHST- 2012/05/31 06:00 [medline]
AID - 10.1111/j.1348-0421.2011.00398.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2012 Jan;56(1):27-39. doi: 10.1111/j.1348-0421.2011.00398.x.