PMID- 22040742
OWN - NLM
STAT- MEDLINE
DCOM- 20120424
LR  - 20230610
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Print)
IS  - 1874-3919 (Linking)
VI  - 75
IP  - 3
DP  - 2012 Jan 4
TI  - Plasma membrane proteomes of differentially matured dendritic cells identified by 
      LC-MS/MS combined with iTRAQ labelling.
PG  - 938-48
LID - 10.1016/j.jprot.2011.10.010 [doi]
AB  - Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but 
      it is unclear what molecular events occur when DCs generate Th2-type responses. 
      Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and 
      pro-Th2 DCs and used a combination of iTRAQ labelling and LC-MS/MS to quantify 
      changes in the proteomes. Analysis was performed on triplicate biological samples 
      and changes verified by flow cytometry. MHC class II molecules and CD29 were 
      up-regulated in pro-Th1 DCs whilst CD18 and CD44 were up-regulated in pro-Th2 
      DCs. One of the most down-regulated molecules in pro-Th1 DCs was YM-1 whilst the 
      greatest decrease in pro-Th2 DCs was NAP-22. Other molecules up-regulated in 
      pro-Th2 DC compared to pro-Th1 DCs included some potentially involved in protein 
      folding during antigen processing (clathrin and Rab-7), whilst other non-membrane 
      proteins such as enzymes/transporters related to cell metabolism (malate 
      dehydrogenase, pyruvate kinase, and ATPase Na(+)/K(+)) were also recorded. This 
      suggests that pro-Th2 DCs are more metabolically active while pro-Th1 DCs have a 
      mature 'end state'. Overall, although several molecules were preferentially 
      expressed on pro-Th2 DCs, our proteomics data support the view of a 'limited 
      maturation' of pro-Th2 DCs compared to pro-Th1 DCs.
CI  - Crown Copyright (c) 2011. Published by Elsevier B.V. All rights reserved.
FAU - Ferret-Bernard, Stephanie
AU  - Ferret-Bernard S
AD  - Department of Biology, University of York, York, YO10 5DD, UK.
FAU - Castro-Borges, William
AU  - Castro-Borges W
FAU - Dowle, Adam A
AU  - Dowle AA
FAU - Sanin, David E
AU  - Sanin DE
FAU - Cook, Peter C
AU  - Cook PC
FAU - Turner, Joseph D
AU  - Turner JD
FAU - MacDonald, Andrew S
AU  - MacDonald AS
FAU - Thomas, Jerry R
AU  - Thomas JR
FAU - Mountford, Adrian P
AU  - Mountford AP
LA  - eng
GR  - G0701437/MRC_/Medical Research Council/United Kingdom
GR  - 071762/WT_/Wellcome Trust/United Kingdom
GR  - BBS/B/08531/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - 095831/WT_/Wellcome Trust/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 072255/WT_/Wellcome Trust/United Kingdom
GR  - BB/C516328/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111025
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cell Membrane/immunology/*metabolism
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Membrane Proteins/immunology/*metabolism
MH  - Mice
MH  - Proteome/immunology/*metabolism
MH  - Proteomics/methods
MH  - Th1 Cells/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
PMC - PMC3444755
EDAT- 2011/11/02 06:00
MHDA- 2012/04/25 06:00
PMCR- 2012/01/04
CRDT- 2011/11/02 06:00
PHST- 2011/08/08 00:00 [received]
PHST- 2011/10/07 00:00 [revised]
PHST- 2011/10/17 00:00 [accepted]
PHST- 2011/11/02 06:00 [entrez]
PHST- 2011/11/02 06:00 [pubmed]
PHST- 2012/04/25 06:00 [medline]
PHST- 2012/01/04 00:00 [pmc-release]
AID - S1874-3919(11)00496-9 [pii]
AID - 10.1016/j.jprot.2011.10.010 [doi]
PST - ppublish
SO  - J Proteomics. 2012 Jan 4;75(3):938-48. doi: 10.1016/j.jprot.2011.10.010. Epub 
      2011 Oct 25.