PMID- 22041752
OWN - NLM
STAT- MEDLINE
DCOM- 20120221
LR  - 20131121
IS  - 2521-9855 (Electronic)
IS  - 0127-5720 (Linking)
VI  - 28
IP  - 2
DP  - 2011 Aug
TI  - Scanning electron microscopic evaluation of the successful sterilization of 
      Lucilia cuprina (Wiedemann) utilized in maggot debridement therapy (mdt).
PG  - 325-32
AB  - In Malaysia, maggot debridement therapy (MDT) utilizes maggots of Lucilia cuprina 
      (Wiedemann) to debride necrotic tissue from wound surface, reduce bacterial 
      infection and therefore, enhance wound healing process. To evaluate the sterility 
      of the sterile maggots produced after sterilization process before delivering 
      onto patient wounds. Sterility of sterile maggots is crucial in ensuring the safe 
      usage of MDT and patient's health. Eggs of L. cuprina collected from a laboratory 
      colony were divided into treated group (sterilized) and control group 
      (non-sterilized). Treated group underwent sterilization while eggs from control 
      group were allowed to hatch without sterilization. Sodium hypochlorite and 
      formaldehyde were the main disinfectants used in this sterilization process. 
      Scanning electron microscope (SEM) was used to examine and ascertain the 
      sterility of sterile maggots. SEM results showed that all sterilized L. cuprina 
      eggs and maggots achieved sterility and all were cleared from bacterial 
      contamination. In contrast, all non-sterilized eggs and maggots were found to be 
      colonized by microorganisms. Sterilization method employed to sterilize eggs and 
      maggots used in Malaysia MDT was proven successful and MDT is safe to be used as 
      wound management tools.
FAU - Yeong, Y S
AU  - Yeong YS
AD  - Medical Entomology Unit, Infectious Diseases Research Centre (IDRC), Institute 
      for Medical Research, Kuala Lumpur, Malaysia.
FAU - Nazni, W A
AU  - Nazni WA
FAU - Santana, R L
AU  - Santana RL
FAU - Mohd Noor, I
AU  - Mohd Noor I
FAU - Lee, H L
AU  - Lee HL
FAU - Mohd Sofian, A
AU  - Mohd Sofian A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Malaysia
TA  - Trop Biomed
JT  - Tropical biomedicine
JID - 8507086
RN  - 1HG84L3525 (Formaldehyde)
RN  - DY38VHM5OD (Sodium Hypochlorite)
SB  - IM
MH  - Animals
MH  - Debridement/methods
MH  - Diptera/*physiology/*ultrastructure
MH  - Formaldehyde/toxicity
MH  - Humans
MH  - Infertility
MH  - Larva/physiology/ultrastructure
MH  - Malaysia
MH  - Microscopy, Electron, Scanning
MH  - Sodium Hypochlorite/toxicity
MH  - Sterilization, Reproductive/methods
MH  - Zygote/drug effects/ultrastructure
EDAT- 2011/11/02 06:00
MHDA- 2012/02/22 06:00
CRDT- 2011/11/02 06:00
PHST- 2011/11/02 06:00 [entrez]
PHST- 2011/11/02 06:00 [pubmed]
PHST- 2012/02/22 06:00 [medline]
PST - ppublish
SO  - Trop Biomed. 2011 Aug;28(2):325-32.