PMID- 22043908
OWN - NLM
STAT- MEDLINE
DCOM- 20111221
LR  - 20211020
IS  - 1528-7394 (Print)
IS  - 1087-2620 (Electronic)
IS  - 0098-4108 (Linking)
VI  - 74
IP  - 22-24
DP  - 2011
TI  - Development of monoclonal antibodies specific for glycated prion protein.
PG  - 1469-75
LID - 10.1080/15287394.2011.618976 [doi]
AB  - Transmissive spongiform encephalopathies (TSE) are neurodegenerative diseases 
      characterized by depositions of abnormally folded prion protein (PrP(TSE)) in 
      brain. PrP(TSE) is at present the only specific biochemical marker of human and 
      animal TSE. As deposits of PrP(TSE) remain in the body for long periods, there is 
      substantial chance of them being nonenzymatically modified by glycation. The 
      detection of glycated PrP(TSE) may have potential to serve as a diagnostic 
      marker. Monoclonal antibodies specific for carboxymethyl lysine/arginine-modified 
      prion protein were prepared. Recombinant human prion protein (rhPrP) was 
      bacterially expressed and purified by affinity chromatography. rhPrP was modified 
      by glyoxylic acid that introduces carboxymethyl groups on lysine and arginine 
      residues present within the molecule of the protein. Modified rhPrP (rhPrP-CML) 
      was used for immunization of 6 mice, and 960 hybridoma cells were prepared. 
      Screening of cell supernatants resulted in the selection of four promising 
      clones. One of them (EM-31) strongly reacts with human and mouse recombinant 
      PrP-CML, and three other clones react also with CML in vitro modified human and 
      mouse brain PrP. Besides possible implication in TSE diagnostics, the antibodies 
      may serve as tolls to advance our knowledge regarding the role of glycation in 
      the prion pathophysiology.
FAU - Dvorakova, Eva
AU  - Dvorakova E
AD  - Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles 
      University-Prague, Studnickova 7, Prague 2, Czech Republic.
FAU - Prouza, Marek
AU  - Prouza M
FAU - Janouskova, Olga
AU  - Janouskova O
FAU - Panigaj, Martin
AU  - Panigaj M
FAU - Holada, Karel
AU  - Holada K
LA  - eng
GR  - F32 NS010335/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptides)
RN  - 0 (Prions)
RN  - 0 (Recombinant Proteins)
RN  - 70YDX3Z2O7 (N(6)-carboxymethyllysine)
RN  - 94ZLA3W45F (Arginine)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal/immunology/metabolism
MH  - Arginine/analogs & derivatives/chemistry/metabolism
MH  - Brain/immunology/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Hybridomas/immunology/metabolism
MH  - Lysine/analogs & derivatives/chemistry/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Peptides/chemistry/metabolism
MH  - Prion Diseases/*diagnosis/immunology/metabolism
MH  - *Prions/chemistry/metabolism
MH  - Recombinant Proteins/chemistry/metabolism
PMC - PMC3259618
EDAT- 2011/11/03 06:00
MHDA- 2011/12/22 06:00
PMCR- 2012/01/17
CRDT- 2011/11/03 06:00
PHST- 2011/11/03 06:00 [entrez]
PHST- 2011/11/03 06:00 [pubmed]
PHST- 2011/12/22 06:00 [medline]
PHST- 2012/01/17 00:00 [pmc-release]
AID - 10.1080/15287394.2011.618976 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2011;74(22-24):1469-75. doi: 
      10.1080/15287394.2011.618976.