PMID- 22047706
OWN - NLM
STAT- MEDLINE
DCOM- 20120411
LR  - 20220316
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 36
IP  - 4
DP  - 2012 Apr
TI  - The utility of fluorescence in situ hybridization analysis in diagnosing 
      myelodysplastic syndromes is limited to cases with karyotype failure.
PG  - 448-52
LID - 10.1016/j.leukres.2011.10.014 [doi]
AB  - Fluorescence in situ hybridization (FISH) is being used increasingly in 
      cytogenetic diagnosis of myelodysplastic syndromes (MDS). However, the utility of 
      FISH in this role has not been well-defined. A total of 249 de novo MDS patients 
      were submitted to karyotyping and FISH analysis for -5/del(5)(q31), 
      -7/del(7)(q31), +8, -17/i(17)(q10), del(20)(q12), and -Y. Of the 234 patients 
      with available karyotypic data, 143 cases (60.9%) demonstrated normal karyotype 
      and 91 cases (39.1%) showed abnormal karyotype. FISH confirmed R-banding findings 
      in 96.6% (226/234) of samples with successful karyotyping and detected 
      cytogenetic abnormalities in 46.7% (7/15) of cases with karyotype failure. Of the 
      3.4% (8/234) patients showing discrepancies between FISH and R-banding, FISH 
      revealed cytogenetic abnormalities in four patients with normal karyotypes and 
      four patients with complex karyotypes. These results highlight FISH analysis has 
      limited value in MDS cases with successful karyotyping and is only informative in 
      MDS cases with karyotype failure.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Jiang, Hui
AU  - Jiang H
AD  - Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of 
      Ministry of Health, The First Affiliated Hospital of Soochow University, 188 
      Shizi Street, Suzhou 215006, Jiangsu, PR China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Wang, Qinrong
AU  - Wang Q
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wu, Yafang
AU  - Wu Y
FAU - Zhang, Jun
AU  - Zhang J
FAU - Bai, Shuxiao
AU  - Bai S
FAU - Wang, Qian
AU  - Wang Q
FAU - He, Guangsheng
AU  - He G
FAU - Sun, Aining
AU  - Sun A
FAU - Wu, Depei
AU  - Wu D
FAU - Chen, Suning
AU  - Chen S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111101
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*diagnosis/*genetics
MH  - Young Adult
EDAT- 2011/11/04 06:00
MHDA- 2012/04/12 06:00
CRDT- 2011/11/04 06:00
PHST- 2011/07/26 00:00 [received]
PHST- 2011/10/13 00:00 [revised]
PHST- 2011/10/15 00:00 [accepted]
PHST- 2011/11/04 06:00 [entrez]
PHST- 2011/11/04 06:00 [pubmed]
PHST- 2012/04/12 06:00 [medline]
AID - S0145-2126(11)00511-X [pii]
AID - 10.1016/j.leukres.2011.10.014 [doi]
PST - ppublish
SO  - Leuk Res. 2012 Apr;36(4):448-52. doi: 10.1016/j.leukres.2011.10.014. Epub 2011 
      Nov 1.