PMID- 22050091
OWN - NLM
STAT- MEDLINE
DCOM- 20120316
LR  - 20211203
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 38
IP  - 12
DP  - 2011 Dec
TI  - Use of classic and novel immunohistochemical markers in the diagnosis of 
      cutaneous myeloid sarcoma.
PG  - 945-53
LID - 10.1111/j.1600-0560.2011.01809.x [doi]
AB  - Cutaneous myeloid sarcoma is often challenging to diagnose based solely upon 
      histopathological features. Although immunohistochemistry can aid in its 
      diagnosis, specific markers have not been clearly identified. We evaluated the 
      utility of immunohistochemical markers in 57 cutaneous myeloid sarcoma cases. In 
      addition to classical markers (CD117, CD163, CD34, myeloperoxidase and lysozyme), 
      we used CD33 and CD14, recently described markers in paraffin-embedded tissue 
      samples, and Kruppel-like factor 4 (KLF-4), a novel monocytic marker. Our results 
      show that lysozyme was expressed in 91%, CD33 in 60%, myeloperoxidase in 54%, 
      CD34 in 39% and CD117 in 36% of cases. An antibody panel that included lysozyme, 
      CD117 and CD33 identified all cases. The monocytic markers CD14, KLF-4 and CD163 
      were expressed in 60, 58 and 40% of all cases, respectively. CD14 and KLF-4 
      expression was significantly more common in cases with monocytic differentiation. 
      CD14 is the single most sensitive and specific marker for monocytic 
      differentiation (79 and 80%). Although KLF-4 in isolation is relatively 
      insensitive (50 and 87%), it enhances sensitivity in detecting monocytic 
      cutaneous myeloid sarcoma when combined with CD14. Our results indicate that in 
      addition to classical immunohistochemical markers, targeted use of newer 
      antibodies, including CD33, CD14 and KLF-4 is useful in the diagnosis of 
      cutaneous myeloid sarcoma and in the detection of monocytic differentiation.
CI  - Copyright (c) 2011 John Wiley & Sons A/S.
FAU - Amador-Ortiz, Catalina
AU  - Amador-Ortiz C
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      Saint Louis, MO, USA.
FAU - Hurley, Maria Y
AU  - Hurley MY
FAU - Ghahramani, Grant K
AU  - Ghahramani GK
FAU - Frisch, Stephanie
AU  - Frisch S
FAU - Klco, Jeffery M
AU  - Klco JM
FAU - Lind, Anne C
AU  - Lind AC
FAU - Nguyen, Tudung T
AU  - Nguyen TT
FAU - Hassan, Anjum
AU  - Hassan A
FAU - Kreisel, Friederike H
AU  - Kreisel FH
FAU - Frater, John L
AU  - Frater JL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Infant
MH  - Kruppel-Like Factor 4
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*metabolism
MH  - Sarcoma, Myeloid/*metabolism/*pathology
MH  - Skin Neoplasms/*metabolism/*pathology
EDAT- 2011/11/05 06:00
MHDA- 2012/03/17 06:00
CRDT- 2011/11/05 06:00
PHST- 2011/11/05 06:00 [entrez]
PHST- 2011/11/05 06:00 [pubmed]
PHST- 2012/03/17 06:00 [medline]
AID - 10.1111/j.1600-0560.2011.01809.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 2011 Dec;38(12):945-53. doi: 10.1111/j.1600-0560.2011.01809.x.