PMID- 22052344
OWN - NLM
STAT- MEDLINE
DCOM- 20120330
LR  - 20211130
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 5
IP  - 2
DP  - 2012 Feb
TI  - Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma 
      cells.
PG  - 383-7
LID - 10.3892/mmr.2011.663 [doi]
AB  - The purpose of this study was to investigate the roles of bcl-2 chromosomal 
      translocation and Bcl-2 protein expression in follicular lymphoma (FL) minimal 
      bone marrow (BM) infiltration. We identified the same bcl-2/IgH fusion gene in 
      paraffin-embedded lymph node (LN) samples and BM samples using 
      immunohistochemistry (IHC), immunocytochemistry (ICC), cytologic morphology and 
      fluorescence in situ hybridization (FISH). The presence of the Bcl-2/IgH fusion 
      gene in the BM samples and paraffin-embedded LN samples from 56 patients with 
      follicular lymphomas was detected using FISH. The Bcl-2 protein levels in BM and 
      paraffin-embedded tissues were quantified using ICC and IHC, respectively. 
      Approximately 78.6% (44/56) of the paraffin‑embedded LN tissue sections that 
      underwent FISH analysis had a bcl-2/IgH translocation. The primary lesion was 
      also positive for the bcl-2/IgH fusion gene, as were the BM minimal infiltrates. 
      The bcl-2/IgH rearrangement occurred in 88.6% (39/44) of the BM specimens. The 
      bcl-2/IgH recombination rate in stage III/IV cancers was significantly different 
      to that observed in stage I/II cancers (p=0.041). In 59% (23/39) of the cases 
      with t(14;18), Bcl-2 was found to be present as assessed by ICC. Positive Bcl-2 
      ICC staining and the t(14;18) translocation (as detected using FISH) were 
      positively correlated (p=0.028). We then applied the FISH method to slides that 
      had previously been morphologically evaluated using Wright-Giemsa staining; any 
      slides with at least one abnormal cell were subjected to FISH analysis following 
      staining. The assessment of bcl-2/IgH translocation status may contribute to the 
      better detection of minimal BM infiltration by FL cells. Utilizing FISH and 
      cytologic morphology techniques allows for earlier and more accessible BM 
      examination.
FAU - Che, Yi-Qun
AU  - Che YQ
AD  - Clinical Laboratory Department, Chao-Yang Hospital, Capital Medical University, 
      Beijing 100020, PR China.
FAU - Liu, Peng
AU  - Liu P
FAU - Wang, Yue
AU  - Wang Y
FAU - Zhang, Chang-Gong
AU  - Zhang CG
FAU - Han, Ya-Ling
AU  - Han YL
FAU - Shen, Di
AU  - Shen D
FAU - Zhang, Ying
AU  - Zhang Y
FAU - Zheng, Cui-Ling
AU  - Zheng CL
FAU - Qi, Jun
AU  - Qi J
FAU - Wang, Qing-Tao
AU  - Wang QT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111103
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells/cytology/metabolism/*pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 18
MH  - Cohort Studies
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/*physiopathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism
MH  - Translocation, Genetic
OTO - NOTNLM
OT  - follicular lymphoma
OT  - fluorescence in situ hybridization
OT  - Bcl-2/IgH
OT  - bone marrow
OT  - infiltration
EDAT- 2011/11/05 06:00
MHDA- 2012/03/31 06:00
CRDT- 2011/11/05 06:00
PHST- 2011/05/04 00:00 [received]
PHST- 2011/08/08 00:00 [accepted]
PHST- 2011/11/05 06:00 [entrez]
PHST- 2011/11/05 06:00 [pubmed]
PHST- 2012/03/31 06:00 [medline]
AID - 10.3892/mmr.2011.663 [doi]
PST - ppublish
SO  - Mol Med Rep. 2012 Feb;5(2):383-7. doi: 10.3892/mmr.2011.663. Epub 2011 Nov 3.