PMID- 22052503
OWN - NLM
STAT- MEDLINE
DCOM- 20120517
LR  - 20111104
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 796
DP  - 2012
TI  - The advantage of global fitting of data involving complex linked reactions.
PG  - 399-421
LID - 10.1007/978-1-61779-334-9_22 [doi]
AB  - In this chapter, we demonstrate the advantage of the simultaneous multicurve 
      nonlinear least-squares analysis over that of the conventional single-curve 
      analysis. Fitting results are subjected to thorough Monte Carlo analysis for 
      rigorous assessment of confidence intervals and parameter correlations. The 
      comparison is performed on a practical example of simulated steady-state reaction 
      kinetics complemented with isothermal calorimetry (ITC) data resembling 
      allosteric behavior of rabbit muscle pyruvate kinase (RMPK). Global analysis 
      improves accuracy and confidence limits of model parameters. Cross-correlation 
      between parameters is also reduced with accompanying enhancement of the 
      model-testing power. This becomes especially important for validation of models 
      with "difficult" highly cross-correlated parameters. We show how proper 
      experimental design and critical evaluation of data can improve the chance of 
      differentiating models.
FAU - Herman, Petr
AU  - Herman P
AD  - Faculty of Mathematics and Physics, Institute of Physics, Charles University, 
      Prague, Czech Republic.
FAU - Lee, J Ching
AU  - Lee JC
LA  - eng
GR  - GM 775551/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Animals
MH  - Calorimetry
MH  - Least-Squares Analysis
MH  - Monte Carlo Method
MH  - Pyruvate Kinase/chemistry/*metabolism
MH  - Rabbits
EDAT- 2011/11/05 06:00
MHDA- 2012/05/18 06:00
CRDT- 2011/11/05 06:00
PHST- 2011/11/05 06:00 [entrez]
PHST- 2011/11/05 06:00 [pubmed]
PHST- 2012/05/18 06:00 [medline]
AID - 10.1007/978-1-61779-334-9_22 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;796:399-421. doi: 10.1007/978-1-61779-334-9_22.