PMID- 22054652
OWN - NLM
STAT- MEDLINE
DCOM- 20120209
LR  - 20161125
IS  - 1552-6259 (Electronic)
IS  - 0003-4975 (Linking)
VI  - 93
IP  - 1
DP  - 2012 Jan
TI  - Novel small-caliber vascular grafts with trimeric Peptide for acceleration of 
      endothelialization.
PG  - 156-63; discussion 163
LID - 10.1016/j.athoracsur.2011.07.055 [doi]
AB  - BACKGROUND: Both rapid endothelialization and the prevention of intimal 
      hyperplasia are essential to improve the patency of small-caliber vascular grafts 
      (SCVGs). Using the peptide array based screening system, we identified the 
      peptide CAG (cysteine-alanine-glycine), which has a high affinity for endothelial 
      cells and a low adhesive property for smooth muscle cells (SMCs). In this 
      article, we report an in vivo analysis of novel vascular grafts that were 
      constructed with a biodegradable polymer (poly-epsilon-caprolactone [PCL]) containing 
      CAG peptide. METHODS: The novel SCVG, which measured 0.7 mm in diameter and 7 mm 
      in length, was fabricated using the electrospinning technique. Carotid arterial 
      replacement was performed on Sprague-Dawley rats using SCVGs with (group CAG) or 
      without CAG (group C). Histologic and biochemical assessments were performed at 
      1, 2, and 6 weeks after implantation. RESULTS: The ratio of endothelialization 
      was significantly higher in group CAG compared with group C (CAG versus C, 
      64.4+/-20.0% versus 42.1+/-8.9% at 1 week; p=0.017; 98.2+/-2.3% versus 72.7+/-12.9% at 2 
      weeks; p=0.001; and 97.4+/-4.6% versus 76.7+/-5.4% at 6 weeks; p<0.001). 
      Additionally, Western blot analysis showed that the level of endothelial nitric 
      oxide synthase (eNOS) at 1 week in group CAG was significantly higher than that 
      in group C (CAG versus C, 1.20+/-0.37 versus 0.34+/-0.16; p=0.013), and that alpha-smooth 
      muscle actin (ASMA) at 6 weeks in group CAG was significantly lower than that in 
      group C (CAG versus C, 0.89+/-0.06 versus 1.25+/-0.22; p=0.04). CONCLUSIONS: The 
      graft with CAG promoted rapid endothelialization and the potential for inhibition 
      of intimal hyperplasia.
CI  - Copyright (c) 2012 The Society of Thoracic Surgeons. Published by Elsevier Inc. All 
      rights reserved.
FAU - Kuwabara, Fumiaki
AU  - Kuwabara F
AD  - Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Narita, Yuji
AU  - Narita Y
FAU - Yamawaki-Ogata, Aika
AU  - Yamawaki-Ogata A
FAU - Kanie, Kei
AU  - Kanie K
FAU - Kato, Ryuji
AU  - Kato R
FAU - Satake, Makoto
AU  - Satake M
FAU - Kaneko, Hiroaki
AU  - Kaneko H
FAU - Oshima, Hideki
AU  - Oshima H
FAU - Usui, Akihiko
AU  - Usui A
FAU - Ueda, Yuichi
AU  - Ueda Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111104
PL  - Netherlands
TA  - Ann Thorac Surg
JT  - The Annals of thoracic surgery
JID - 15030100R
RN  - 0 (Caproates)
RN  - 0 (Lactones)
RN  - 56RE988L1R (caprolactone)
SB  - IM
MH  - Absorbable Implants
MH  - Animals
MH  - Arterial Occlusive Diseases/*surgery
MH  - *Blood Vessel Prosthesis
MH  - *Caproates
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/*pathology
MH  - Hyperplasia
MH  - *Lactones
MH  - Male
MH  - Miniaturization
MH  - Muscle, Smooth, Vascular/*pathology
MH  - Prosthesis Design
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2011/11/08 06:00
MHDA- 2012/02/10 06:00
CRDT- 2011/11/08 06:00
PHST- 2011/05/05 00:00 [received]
PHST- 2011/07/12 00:00 [revised]
PHST- 2011/07/19 00:00 [accepted]
PHST- 2011/11/08 06:00 [entrez]
PHST- 2011/11/08 06:00 [pubmed]
PHST- 2012/02/10 06:00 [medline]
AID - S0003-4975(11)01817-0 [pii]
AID - 10.1016/j.athoracsur.2011.07.055 [doi]
PST - ppublish
SO  - Ann Thorac Surg. 2012 Jan;93(1):156-63; discussion 163. doi: 
      10.1016/j.athoracsur.2011.07.055. Epub 2011 Nov 4.