PMID- 2205543
OWN - NLM
STAT- MEDLINE
DCOM- 19901024
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 91
IP  - 1
DP  - 1990 Jul 2
TI  - Differential gene expression mediated by late, very late and hybrid baculovirus 
      promoters.
PG  - 87-94
AB  - A series of recombinant viruses was constructed to study the regulation of 
      expression from the promoter (Pcap) for the major capsid-protein-encoding gene 
      (vp39) of the baculovirus, Autographa californica nuclear polyhedrosis virus. 
      Each virus of this series contains the cat reporter gene under the control of 
      Pcap or portions thereof. Pcap regulation of cat was determined at the RNA and 
      enzyme activity levels, and was compared to cat gene expression driven by Ppolh, 
      the promoter of the polyhedrin gene (polh). Although optimum expression was 
      achieved with a 464-bp region, which included all three Pcap transcription start 
      points (tsp), a 99-bp segment of Pcap containing a single tsp was sufficient to 
      direct late cat expression. Ppolh and Pcap-mediated regulation differed 
      temporally; Pcap-cat constructs were expressed at 12 h post-infection (p.i.) and 
      continued through 48 h p.i., whereas Ppolh-cat were not expressed at 12 h, but 
      were initiated around 18 h p.i. and underwent a burst of expression between 24 
      and 48 h p.i. A recombinant virus carrying a hybrid and polh promoter (Pcappolh) 
      was also constructed and studied; Pcappolh contained the two distal tsp of Pcap 
      and a proximal tsp of Ppolh. Pcappolh exhibited both 'late' and 'very late' 
      regulation; the distal tsp were regulated as late sites and the proximal tsp was 
      regulated primarily as a very late site although it also showed a weak late 
      response. Hybrid late/very late promoters should prove useful in optimizing 
      foreign gene expression using baculovirus vectors.
FAU - Thiem, S M
AU  - Thiem SM
AD  - Department of Genetics, University of Georgia, Athens 30602.
FAU - Miller, L K
AU  - Miller LK
LA  - eng
GR  - AI 23719/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - *Gene Expression Regulation, Viral
MH  - Insect Viruses/*genetics
MH  - Insecta
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - *Promoter Regions, Genetic
MH  - Recombination, Genetic
MH  - Restriction Mapping
EDAT- 1990/07/02 00:00
MHDA- 1990/07/02 00:01
CRDT- 1990/07/02 00:00
PHST- 1990/07/02 00:00 [pubmed]
PHST- 1990/07/02 00:01 [medline]
PHST- 1990/07/02 00:00 [entrez]
AID - 0378-1119(90)90166-O [pii]
AID - 10.1016/0378-1119(90)90166-o [doi]
PST - ppublish
SO  - Gene. 1990 Jul 2;91(1):87-94. doi: 10.1016/0378-1119(90)90166-o.