PMID- 22057872
OWN - NLM
STAT- MEDLINE
DCOM- 20120430
LR  - 20211021
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 61
IP  - 1
DP  - 2012 Jan
TI  - The effects of leflunomide on CD4(+)CD25 (+)Foxp3 (+) T regulatory cells in mice 
      receiving allogeneic bone marrow transplantation.
PG  - 53-60
LID - 10.1007/s00011-011-0388-4 [doi]
AB  - OBJECTIVE AND DESIGN: Leflunomide (LEF) is effective not only in different animal 
      models of autoimmune diseases and the therapy of patients with rheumatoid 
      arthritisbut also in graft rejection. The effect of LEF on CD4(+)CD25(+)T 
      regulatory cells (Treg) was determined in a mouse model of allogeneic bone marrow 
      transplantation. MATERIAL OR SUBJECTS: BALB/c and C57BL/6 mice were used as 
      donors and recipients, respectively. TREATMENT: C57BL/6 mice were given 2 Gy 
      total-body irradiation, followed by an intravenous injection of 2 x 10(7) BALB/c 
      bone marrow cells (BMCs). Mice were treated with LEF daily at a dose of 30 
      mg/kg/day for 2 weeks. RESULTS: In naive mice, LEF significantly decreased the 
      percentage of CD4(+)CD25(+) Treg cells in spleens (P < 0.05), but not in lymph 
      nodes, though LEF enhanced the percentages of CD4(+)CD25(+) Treg cells in CD4 
      single positive cells in the thymocytes and blood (P < 0.05). Furthermore, LEF 
      significantly decreased the percentage of CD4(+)CD25(+) Treg cells in the spleens 
      of mice that received allogeneic BMCs. CONCLUSIONS: LEF decreases peripheral 
      CD4(+)CD25(+) Treg cells in un-immunizing and immunizing recipients, indicating 
      that LEF might not be an ideal candidate for the treatment of autoimmune diseases 
      or graft rejection with respect to induction of immune tolerance.
FAU - Jin, Di
AU  - Jin D
AD  - Transplantation Biology Research Division, State Key Laboratory of Biomembrane 
      and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 
      Beijing, China.
FAU - Duan, Kaizhong
AU  - Duan K
FAU - Zhang, Lianjun
AU  - Zhang L
FAU - Peng, Jianxia
AU  - Peng J
FAU - Zhao, Yong
AU  - Zhao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111105
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Isoxazoles)
RN  - G162GK9U4W (Leflunomide)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Autoimmune Diseases/metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - CD4-Positive T-Lymphocytes/*cytology
MH  - Female
MH  - Forkhead Transcription Factors/*biosynthesis
MH  - Interleukin-2 Receptor alpha Subunit/*biosynthesis
MH  - Isoxazoles/*pharmacology
MH  - Leflunomide
MH  - Leukocytes, Mononuclear/cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Regulatory/*cytology
EDAT- 2011/11/08 06:00
MHDA- 2012/05/01 06:00
CRDT- 2011/11/08 06:00
PHST- 2011/02/08 00:00 [received]
PHST- 2011/09/16 00:00 [accepted]
PHST- 2011/08/23 00:00 [revised]
PHST- 2011/11/08 06:00 [entrez]
PHST- 2011/11/08 06:00 [pubmed]
PHST- 2012/05/01 06:00 [medline]
AID - 10.1007/s00011-011-0388-4 [doi]
PST - ppublish
SO  - Inflamm Res. 2012 Jan;61(1):53-60. doi: 10.1007/s00011-011-0388-4. Epub 2011 Nov 
      5.