PMID- 22065605
OWN - NLM
STAT- MEDLINE
DCOM- 20120629
LR  - 20191210
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 113
IP  - 3
DP  - 2012 Mar
TI  - In vivo magnetic resonance imaging tracking of SPIO-labeled human umbilical cord 
      mesenchymal stem cells.
PG  - 1005-12
LID - 10.1002/jcb.23432 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) can be efficiently labeled 
      by superparamagnetic iron oxide (SPIO) nanoparticles, which produces low signal 
      intensity on magnetic resonance imaging (MRI) in vitro. This study was to 
      evaluate the feasibility of in vivo tracking for hUC-MSCs labeled by SPIO with 
      noninvasive MRI. SPIO was added to cultures at concentrations equivalent to 0, 7, 
      14, 28, and 56 microg Fe/ml (diluted with DMEM/F12) and incubated for 16 h. Prussian 
      Blue staining was used to determinate the labeling efficiency. Rats were randomly 
      divided into three groups, control group, hUC-MSCs group, and SPIO-labeled 
      hUC-MSCs group. All groups were subjected to spinal cord injury (SCI) by weight 
      drop device. Rats were examined for neurological function. In vivo MRI was used 
      to track SPIO-labeled hUC-MSCs transplanted in rats spinal cord. Survival and 
      migration of hUC-MSCs were also explored using immunofluorescence. Significant 
      improvements in locomotion were observed in the hUC-MSCs groups. There was 
      statistical significance compared with control group. In vivo MRI 1 and 3 weeks 
      after injection showed a large reduction in signal intensity in the region 
      transplanted with SPIO-labeled hUC-MSCs. The images from unlabeled hUC-MSCs 
      showed a smaller reduction in signal intensity. Transplanted hUC-MSCs engrafted 
      within the injured rats spinal cord and survived for at least 8 weeks. In 
      conclusion, hUC-MSCs can survive and migrate in the host spinal cord after 
      transplantation, which promote functional recovery after SCI. Noninvasive imaging 
      of transplanted SPIO-labeled hUC-MSCs is feasible.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Hu, Sheng-Li
AU  - Hu SL
AD  - Department of Neurosurgery, Southwest Hospital, Third Military Medical 
      University, No. 29, GaoTanYan Street, Chong-Qing 400038, PR China.
FAU - Lu, Pei-Gang
AU  - Lu PG
FAU - Zhang, Li-Jun
AU  - Zhang LJ
FAU - Li, Fei
AU  - Li F
FAU - Chen, Zhi
AU  - Chen Z
FAU - Wu, Nan
AU  - Wu N
FAU - Meng, Hui
AU  - Meng H
FAU - Lin, Jiang-Kai
AU  - Lin JK
FAU - Feng, Hua
AU  - Feng H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Magnetite Nanoparticles)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Cell Tracking/*methods
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - *Magnetic Resonance Imaging
MH  - *Magnetite Nanoparticles
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Motor Activity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/physiopathology/therapy
MH  - Umbilical Cord/*cytology
EDAT- 2011/11/09 06:00
MHDA- 2012/06/30 06:00
CRDT- 2011/11/09 06:00
PHST- 2011/11/09 06:00 [entrez]
PHST- 2011/11/09 06:00 [pubmed]
PHST- 2012/06/30 06:00 [medline]
AID - 10.1002/jcb.23432 [doi]
PST - ppublish
SO  - J Cell Biochem. 2012 Mar;113(3):1005-12. doi: 10.1002/jcb.23432.