PMID- 22067315
OWN - NLM
STAT- MEDLINE
DCOM- 20120305
LR  - 20211021
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 153
IP  - 1
DP  - 2012 Jan
TI  - Type 2 corticotropin-releasing factor receptor in the ventromedial nucleus of 
      hypothalamus is critical in regulating feeding and lipid metabolism in white 
      adipose tissue.
PG  - 166-76
LID - 10.1210/en.2011-1312 [doi]
AB  - Ventromedial nucleus of hypothalamus (VMH) plays a critical role in regulating 
      feeding and energy metabolism. The nucleus expresses high levels of the type 2 
      corticotropin-releasing factor receptor (CRFR2) and receives prominent 
      innervation of nerve fibers containing Urocortin 3 (Ucn 3), an endogenous ligand 
      of the receptor. In the present study, we showed that mice deficient in Ucn 3 had 
      elevated basal feeding and increased nocturnal food intake after overnight 
      fasting compared with the wild-type (WT) littermates. The Ucn 3 null mice also 
      had lower circulating insulin levels compared with those of the WT mice. 
      Interestingly, the mutant mice maintained a comparable body weight with the WT 
      littermates. Mice with reduced CRFR2 expression in the VMH by small hairpin RNA 
      knockdown (KD) recapitulated feeding phenotypes observed in the Ucn 3 null mice. 
      However, VMH CRFR2 KD mice gained significantly more weight than control mice. 
      The weight gain was due to an accumulation of white adipose tissue (WAT) 
      accompanied by reduced plasma free fatty acids and glycerol levels, increased 
      respiratory quotients, and improved glucose tolerance. On the other hand, plasma 
      insulin levels were comparable with the receptor KD and control mice. 
      Furthermore, the expression of several genes, including hormone-sensitive lipase, 
      was significantly reduced in the WAT of VMH CRFR2 KD mice compared with controls. 
      These results indicate that Ucn 3 signaling through CRFR2 is a critical molecular 
      mediator in the VMH in regulating feeding and lipid metabolism in WAT.
FAU - Chao, Hongxia
AU  - Chao H
AD  - Department of Pharmacology, University of Virginia, Charlottesville, Virginia 
      22903, USA.
FAU - Digruccio, Michael
AU  - Digruccio M
FAU - Chen, Peilin
AU  - Chen P
FAU - Li, Chien
AU  - Li C
LA  - eng
GR  - R01 DK078049/DK/NIDDK NIH HHS/United States
GR  - R01 DK-078049/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20111108
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Blood Glucose)
RN  - 0 (CRF receptor type 2)
RN  - 0 (Insulin)
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 0 (Urocortins)
SB  - IM
MH  - Adipose Tissue, White/*metabolism
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Body Weight
MH  - Eating/*physiology
MH  - Energy Metabolism
MH  - Fasting
MH  - Gene Knockdown Techniques
MH  - Glucose Tolerance Test
MH  - Insulin/blood
MH  - Insulin Resistance
MH  - *Lipid Metabolism
MH  - Male
MH  - Mice
MH  - Mice, 129 Strain
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Receptors, Corticotropin-Releasing Hormone/antagonists & 
      inhibitors/genetics/*physiology
MH  - Urocortins/deficiency/genetics
MH  - Ventromedial Hypothalamic Nucleus/*physiology
PMC - PMC3249673
EDAT- 2011/11/10 06:00
MHDA- 2012/03/06 06:00
PMCR- 2013/01/01
CRDT- 2011/11/10 06:00
PHST- 2011/11/10 06:00 [entrez]
PHST- 2011/11/10 06:00 [pubmed]
PHST- 2012/03/06 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - en.2011-1312 [pii]
AID - EN-11-1312 [pii]
AID - 10.1210/en.2011-1312 [doi]
PST - ppublish
SO  - Endocrinology. 2012 Jan;153(1):166-76. doi: 10.1210/en.2011-1312. Epub 2011 Nov 
      8.