PMID- 22069985
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 25
IP  - 10
DP  - 2011 Oct
TI  - [Effect of platelet lysate on chondrogenic differentiation of human umbilical 
      cord derived mesenchymal stem cells in vitro].
PG  - 1250-5
AB  - OBJECTIVE: To study the effect of platelet lysate (PL) on chondrogenic 
      differentiation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) 
      in vitro. METHODS: Umbilical cords were voluntarily donated by healthy mothers. 
      The hUCMSCs were isolated by collagenase digestion and cultured in vitro. The 
      surface markers of the cells were detected by flow cytometer. According to 
      different components of inductive medium, the cultured hUCMSCs were divided into 
      3 groups: group A [H-DMEM medium, 10% fetal bovine serum (FBS), and 10%PL]; group 
      B [H-DMEM medium, 10%FBS, 10 ng/mL transforming growth factor beta1 (TGF-beta1), 
      1 x 10(-7) mol/L dexamethasone, 50 microg/mL Vitamin C, and 1% 
      insulin-transferrin-selenium (ITS)]; and group C (H-DMEM medium, 10%FBS, 10 ng/mL 
      TGF-beta1, 1 x 10(-7) mol/L dexamethasone, 50 microg/mL vitamin C, 1%ITS, and 
      10%PL). The hUCMSCs were induced in the mediums for 2 weeks. Toluidine blue 
      staining was used to detect the secretion of chondrocyte matrix. 
      Immunofluorescence method was used to identify the existence of collagen type II. 
      The expressions of Aggrecan and collagen type II were detected by 
      semiquantitative RT-PCR. RESULTS: Flow cytometer results showed that the hUCMSCs 
      did not express the surface markers of hematopoietic cell CD34, CD45, and human 
      leukocyte antigen DR, but expressed the surface markers of adhesion molecule and 
      mesenchymal stem cells CD44, CD105, and CD146. Toluidine blue staining and 
      immunofluorescence showed positive results in group C, weak positive results in 
      group B, and negative results in group A. Semiquantitative RT-PCR showed the 
      expressions of Aggrecan and collagen type II at mRNA level in groups B and C, but 
      no expression in group A. The mRNA expressions of Aggrecan and collagen type II 
      were higher in group C than in group B (P < 0.05). CONCLUSION: Only 10%PL can not 
      induce differentiation of hUCMSCs into chondrocytes, but it can be a supplement 
      to the induced mediums. PL can improve hUCMSCs differentiating into chondrocytes 
      obviously in vitro. This study provides new available conditions for constructing 
      tissue engineered cartilage.
FAU - Feng, Xuetao
AU  - Feng X
AD  - Department of Orthopaedics, Affiliated Hospital of Medical College, Qingdao 
      University, Qingdao Shandong 266000, PR China.
FAU - Tian, Shaoqi
AU  - Tian S
FAU - Sun, Kang
AU  - Sun K
FAU - Zhang, Jihua
AU  - Zhang J
FAU - Zhang, Cailong
AU  - Zhang C
FAU - Liu, Shihai
AU  - Liu S
FAU - Zhou, Ming
AU  - Zhou M
FAU - Lu, Jiangtao
AU  - Lu J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Culture Media)
SB  - IM
MH  - *Blood Platelets
MH  - Bone Marrow Cells/*cytology
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology
MH  - Culture Media
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Tissue Engineering/methods
MH  - Umbilical Cord/cytology
EDAT- 2011/11/11 06:00
MHDA- 2012/05/11 06:00
CRDT- 2011/11/11 06:00
PHST- 2011/11/11 06:00 [entrez]
PHST- 2011/11/11 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Oct;25(10):1250-5.