PMID- 22072352
OWN - NLM
STAT- MEDLINE
DCOM- 20120312
LR  - 20200204
IS  - 1699-5198 (Electronic)
IS  - 0212-1611 (Linking)
VI  - 26
IP  - 5
DP  - 2011 Sep-Oct
TI  - [Effect of dietary fiber in the quantitative expression of butyrate receptor 
      GPR43 in rats colon].
PG  - 1052-8
LID - S0212-16112011000500020 [pii]
LID - 10.1590/S0212-16112011000500020 [doi]
AB  - INTRODUCTION: Short chain fatty acids (SCFA) acetate, propionate and butyrate are 
      the major anions produced by the bacterial fermentation of dietary fiber (DF) in 
      colon. Recently, butyrate has been recently studied because is important to 
      maintain colonic functions and because it has been related with a protective 
      effect in colorectal cancer, which is mainly, explained by its potential to 
      regulate gene expression by inhibiting enzyme histonedeacetylase (HDAC). Several 
      investigationsshown that SCFAreceptor GPR43 is involved insignal transduction 
      mechanisms once they bind to ligands such as butyrate to generate different 
      physiological effects in colonocytes. OBJECTIVE: Determine if dietary fiber 
      consumption from nopal (Opuntia ficus I.) containing a ratio of soluble-insoluble 
      fiber 40/60, has a direct influence on the quantitative expression of 
      butyrate-specific receptor GPR43. METHODS: Wistar rats were fed with four 
      different diets formulated at different concentrations of dietary fiber of 0, 5, 
      15 and 25% of dietary fiber from opuntia, respectively. RESULTS AND DISCUSSION: 
      The results shown an increase in the expression of GPR43 (93.1%) when rats was 
      fed with a 5% fiber diet, using beta-actin as a reference gene. The results of this 
      investigation will contribute to determinate the relation of diet with intestinal 
      health for the purpose of expanding the knowledge of butyric acid on colonic 
      functions.
FAU - Corte Osorio, L Y
AU  - Corte Osorio LY
AD  - Programa Institucional de Maestria en Ciencias Biologicas, Universidad Michoacana 
      de San Nicolas de Hidalgo, Mexico.
FAU - Martinez Flores, H E
AU  - Martinez Flores HE
FAU - Ortiz Alvarado, R
AU  - Ortiz Alvarado R
LA  - spa
PT  - Journal Article
TT  - Efecto del consumo de la fibra dietetica en la expresion cuantitativa del 
      receptor de butirato GPR43 en colon de ratas.
PL  - Spain
TA  - Nutr Hosp
JT  - Nutricion hospitalaria
JID - 9100365
RN  - 0 (Actins)
RN  - 0 (Butyrates)
RN  - 0 (Dietary Fiber)
RN  - 0 (Ffar2 protein, rat)
RN  - 0 (Ligands)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Butyrates/*metabolism
MH  - Colon/cytology/drug effects/*metabolism
MH  - Diet
MH  - Dietary Fiber/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enterocytes/drug effects/metabolism
MH  - Fermentation
MH  - Fluorescence
MH  - Ligands
MH  - Opuntia
MH  - RNA/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, G-Protein-Coupled/*biosynthesis/*genetics
EDAT- 2011/11/11 06:00
MHDA- 2012/03/13 06:00
CRDT- 2011/11/11 06:00
PHST- 2010/10/10 00:00 [received]
PHST- 2011/03/04 00:00 [accepted]
PHST- 2011/11/11 06:00 [entrez]
PHST- 2011/11/11 06:00 [pubmed]
PHST- 2012/03/13 06:00 [medline]
AID - S0212-16112011000500020 [pii]
AID - 10.1590/S0212-16112011000500020 [doi]
PST - ppublish
SO  - Nutr Hosp. 2011 Sep-Oct;26(5):1052-8. doi: 10.1590/S0212-16112011000500020.