PMID- 22072755
OWN - NLM
STAT- MEDLINE
DCOM- 20120222
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 2
DP  - 2012 Jan
TI  - Viral adaptation to host immune responses occurs in chronic hepatitis B virus 
      (HBV) infection, and adaptation is greatest in HBV e antigen-negative disease.
PG  - 1181-92
LID - 10.1128/JVI.05308-11 [doi]
AB  - Hepatitis B virus (HBV)-specific T-cell responses are important in the natural 
      history of HBV infection. The number of known HBV-specific T-cell epitopes is 
      limited, and it is not clear whether viral evolution occurs in chronic HBV 
      infection. We aimed to identify novel HBV T-cell epitopes by examining the 
      relationship between HBV sequence variation and the human leukocyte antigen (HLA) 
      type in a large prospective clinic-based cohort of Asian patients with chronic 
      HBV infection recruited in Australia and China (n = 119). High-resolution 4-digit 
      HLA class I and II typing and full-length HBV sequencing were undertaken for 
      treatment-naive individuals (52% with genotype B, 48% with genotype C, 63% HBV e 
      antigen [HBeAg] positive). Statistically significant associations between HLA 
      types and HBV sequence variation were identified (n = 49) at 41 sites in the HBV 
      genome. Using prediction programs, we determined scores for binding between 
      peptides containing these polymorphisms and associated HLA types. Among the 
      regions that could be tested, HLA binding was predicted for 14/18 (78%). We 
      identified several HLA-associated polymorphisms involving likely known anchor 
      residues that resulted in altered predicted binding scores. Some HLA-associated 
      polymorphisms fell within known T-cell epitopes with matching HLA restriction. 
      Enhanced viral adaptation (defined as the presence of the relevant HLA and the 
      escaped amino acid) was independently associated with HBeAg-negative disease (P = 
      0.003). Thus, HBV appears to be under immune pressure in chronic HBV infection, 
      particularly in HBeAg-negative disease.
FAU - Desmond, Christopher P
AU  - Desmond CP
AD  - Department of Gastroenterology, The Alfred, Melbourne, Australia.
FAU - Gaudieri, Silvana
AU  - Gaudieri S
FAU - James, Ian R
AU  - James IR
FAU - Pfafferott, Katja
AU  - Pfafferott K
FAU - Chopra, Abha
AU  - Chopra A
FAU - Lau, George K
AU  - Lau GK
FAU - Audsley, Jennifer
AU  - Audsley J
FAU - Day, Caroline
AU  - Day C
FAU - Chivers, Sarah
AU  - Chivers S
FAU - Gordon, Adam
AU  - Gordon A
FAU - Revill, Peter A
AU  - Revill PA
FAU - Bowden, Scott
AU  - Bowden S
FAU - Ayres, Anna
AU  - Ayres A
FAU - Desmond, Paul V
AU  - Desmond PV
FAU - Thompson, Alexander J
AU  - Thompson AJ
FAU - Roberts, Stuart K
AU  - Roberts SK
FAU - Locarnini, Stephen A
AU  - Locarnini SA
FAU - Mallal, Simon A
AU  - Mallal SA
FAU - Lewin, Sharon R
AU  - Lewin SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111109
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Hepatitis B e Antigens)
SB  - IM
MH  - Adaptation, Biological
MH  - Amino Acid Sequence
MH  - Australia
MH  - China
MH  - Epitopes, T-Lymphocyte/genetics/immunology
MH  - Female
MH  - HLA Antigens/*genetics/*immunology
MH  - Hepatitis B e Antigens/*blood/genetics/immunology
MH  - Hepatitis B virus/classification/genetics/immunology/*physiology
MH  - Hepatitis B, Chronic/genetics/*immunology/*virology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - Prospective Studies
PMC - PMC3255822
EDAT- 2011/11/11 06:00
MHDA- 2012/02/23 06:00
PMCR- 2012/07/01
CRDT- 2011/11/11 06:00
PHST- 2011/11/11 06:00 [entrez]
PHST- 2011/11/11 06:00 [pubmed]
PHST- 2012/02/23 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - JVI.05308-11 [pii]
AID - 5308-11 [pii]
AID - 10.1128/JVI.05308-11 [doi]
PST - ppublish
SO  - J Virol. 2012 Jan;86(2):1181-92. doi: 10.1128/JVI.05308-11. Epub 2011 Nov 9.