PMID- 2207334
OWN - NLM
STAT- MEDLINE
DCOM- 19901119
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 76
IP  - 8
DP  - 1990 Oct 15
TI  - The role of cellular maturation in neutrophil heterogeneity.
PG  - 1639-46
AB  - Previous studies have shown that many neutrophil (PMN) characteristics are 
      heterogeneous but the origin of PMN heterogeneity is unknown. It is unclear if 
      PMN functional heterogeneity is secondary to maturational differences or due to 
      distinct subpopulations of cells that possess different functional capacities. 
      The PMN 31D8 antigen is a useful probe for evaluation of PMN subpopulations. The 
      majority of PMNs (approximately 85%) exhibit a high intensity fluorescence after 
      31D8 monoclonal antibody (MoAb) labeling (31D8 enriched or "bright" PMNs) as 
      determined by flow cytometric analysis. These cells are more functional than 
      cells with low intensity fluorescence (31D8 diminished or "dull" PMNs). Various 
      immunologic, clonogenic and functional techniques were used to study the 
      expression of the 31D8 antigen in HL-60 cells and myeloid cells in order to 
      evaluate antigenic and functional heterogeneity during morphologic maturation. 
      The results of this study indicate that the percentage of 31D8 antigen positive 
      (31D8 antigen enriched and diminished) bone marrow cells increases from 20 +/- 
      11% in myeloblast cells to 68 +/- 10% in promyelocytes, 93 +/- 2% in myelocytes 
      and 99 +/- 1% in bands and PMNs. 31D8 antigen enriched cells first appear at the 
      myelocyte stage (32 +/- 10%) and increase in bands (52 +/- 13%), marrow PMNs (62 
      +/- 13%) and peripheral blood PMNs (88 +/- 4%). These data indicate that the 
      heterogeneous expression of 31D8 antigen in PMNs is due, at least in part, to 
      maturational differences within the PMN population and raise the possibility that 
      other heterogeneously expressed PMN characteristics are also maturationally 
      derived. They also suggest that 31D8 antigenic expression may be a more precise 
      indicator of myeloid functional maturation than maturation as identified by 
      cellular morphology.
FAU - Krause, P J
AU  - Krause PJ
AD  - Department of Pediatrics, Hartford Hospital, CT 06115.
FAU - Todd, M B
AU  - Todd MB
FAU - Hancock, W W
AU  - Hancock WW
FAU - Pastuszak, W T
AU  - Pastuszak WT
FAU - Maderazo, E G
AU  - Maderazo EG
FAU - Hild, D H
AU  - Hild DH
FAU - Kosciol, C M
AU  - Kosciol CM
LA  - eng
GR  - CA 08341/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Antigens, Surface/analysis
MH  - Bone Marrow Cells
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Histocytochemistry
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunophenotyping
MH  - Leukemia, Promyelocytic, Acute/immunology/pathology
MH  - Neutrophils/*cytology/immunology
MH  - Tumor Cells, Cultured
EDAT- 1990/10/15 00:00
MHDA- 1990/10/15 00:01
CRDT- 1990/10/15 00:00
PHST- 1990/10/15 00:00 [pubmed]
PHST- 1990/10/15 00:01 [medline]
PHST- 1990/10/15 00:00 [entrez]
AID - S0006-4971(20)75238-9 [pii]
PST - ppublish
SO  - Blood. 1990 Oct 15;76(8):1639-46.