PMID- 22074805
OWN - NLM
STAT- MEDLINE
DCOM- 20120719
LR  - 20120206
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 43
IP  - 1
DP  - 2012 Jan
TI  - L-amino acid decarboxylase- and tyrosine hydroxylase-immunoreactive cells in the 
      extended olfactory amygdala and elsewhere in the adult prairie vole brain.
PG  - 76-85
LID - 10.1016/j.jchemneu.2011.10.006 [doi]
AB  - Neurons synthesizing dopamine (DA) are widely distributed in the brain and 
      implicated in a tremendous number of physiological and behavioral functions, 
      including socioreproductive behaviors in rodents. We have recently been 
      investigating the possible involvement of sex- and species-specific 
      TH-immunoreactive (TH-ir) cells in the male prairie vole (Microtus ochrogaster) 
      principal bed nucleus of the stria terminalis (pBST) and posterodorsal medial 
      amygdala (MeApd) in the chemosensory control of their monogamous pairbonding and 
      parenting behaviors. These TH-ir cells are not immunoreactive for 
      dopamine-beta-hydroxylase (DBH), suggesting they are not noradrenergic but 
      possibly DAergic. A DAergic phenotype would require them to contain aromatic 
      L-amino acid decarboxylase (AADC) and here we examined the existence of cells 
      immunoreactive for both TH and AADC in the pBST and MeApd of adult virgin male 
      and female prairie voles. We also investigated the presence of TH/AADC cells in 
      the anteroventral periventricular nucleus (AVPV), medial preoptic area (MPO), 
      arcuate nucleus (ARH), zona incerta (ZI), substantia nigra (SN) and ventral 
      tegmental area (VTA). Among our findings were: (1) the pBST and MeApd each 
      contained completely non-overlapping distributions of TH-ir and AADC-ir cells, 
      (2) the AVPV contained surprisingly few AADC-ir cells and almost no TH-ir cells 
      contained AADC-ir, (3) approximately 60% of the TH-ir cells in the MPO, ARH, and 
      ZI also contained AADC-ir, (4) unexpectedly, only about half of TH-ir cells in 
      the SN and VTA contained AADC-ir, and (5) notable populations of AADC-ir cells 
      were found outside traditional monoamine-synthesizing regions, including some 
      sites that do not contain AADC-ir cells in adult laboratory rats or cats (medial 
      septum and cerebral cortex). In the absence of the chemical requirements to 
      produce DA, monoenzymatic TH-ir cells in the virgin adult prairie vole pBST, 
      MeApd, and elsewhere in their brain may instead produce L-DOPA as an end product 
      and use it as a neurotransmitter or neuromodulator, similar to what has been 
      observed for monoenzymatic TH-synthesizing cells in the laboratory rat brain.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Ahmed, Eman I
AU  - Ahmed EI
AD  - Neuroscience Program, 108 Giltner Hall, Michigan State University, East Lansing, 
      MI 48824, USA.
FAU - Northcutt, Katharine V
AU  - Northcutt KV
FAU - Lonstein, Joseph S
AU  - Lonstein JS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111102
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
SB  - IM
MH  - Amygdala/*metabolism
MH  - Animals
MH  - Aromatic-L-Amino-Acid Decarboxylases/*metabolism
MH  - Arvicolinae/*metabolism
MH  - Brain/metabolism
MH  - Female
MH  - Male
MH  - Neurons/metabolism
MH  - Olfactory Pathways/*metabolism
MH  - Tyrosine 3-Monooxygenase/*metabolism
EDAT- 2011/11/15 06:00
MHDA- 2012/07/20 06:00
CRDT- 2011/11/15 06:00
PHST- 2011/08/30 00:00 [received]
PHST- 2011/10/25 00:00 [revised]
PHST- 2011/10/26 00:00 [accepted]
PHST- 2011/11/15 06:00 [entrez]
PHST- 2011/11/15 06:00 [pubmed]
PHST- 2012/07/20 06:00 [medline]
AID - S0891-0618(11)00178-5 [pii]
AID - 10.1016/j.jchemneu.2011.10.006 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2012 Jan;43(1):76-85. doi: 10.1016/j.jchemneu.2011.10.006. Epub 
      2011 Nov 2.