PMID- 22075625
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20211021
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 27
IP  - 1
DP  - 2012
TI  - Comparison among amoA primers suited for quantification and diversity analyses of 
      ammonia-oxidizing bacteria in soil.
PG  - 94-8
AB  - Ammonia monooxygenase subunit A gene (amoA) is frequently used as a functional 
      gene marker for diversity analysis of ammonia-oxidizing bacteria (AOB). To select 
      a suitable amoA primer for real-time PCR and PCR-denaturing gradient gel 
      electrophoresis (DGGE), three reverse primers (degenerate primer amoA-2R; 
      non-degenerate primers amoA-2R-GG and amoA-2IR) were examined. No significant 
      differences were observed among the three primers in terms of quantitative values 
      of amoA from environmental samples using real-time PCR. We found that PCR-DGGE 
      analysis with the amoA-2IR primer gave the best results in this studied soil. 
      These results indicate that amoA-2IR is a suitable primer for community analysis 
      of AOB in the environment.
FAU - Shimomura, Yumi
AU  - Shimomura Y
AD  - National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan. 
      yshimo@affrc.go.jp
FAU - Morimoto, Sho
AU  - Morimoto S
FAU - Takada Hoshino, Yuko
AU  - Takada Hoshino Y
FAU - Uchida, Yoshitaka
AU  - Uchida Y
FAU - Akiyama, Hiroko
AU  - Akiyama H
FAU - Hayatsu, Masahito
AU  - Hayatsu M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111110
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Bacteria/classification/enzymology/genetics/*isolation & purification
MH  - Bacterial Proteins/*genetics
MH  - *Biodiversity
MH  - DNA Primers/*genetics
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Oxidoreductases/*genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - *Soil Microbiology
PMC - PMC4036032
EDAT- 2011/11/15 06:00
MHDA- 2012/09/11 06:00
PMCR- 2012/03/01
CRDT- 2011/11/15 06:00
PHST- 2011/11/15 06:00 [entrez]
PHST- 2011/11/15 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - JST.JSTAGE/jsme2/ME11230 [pii]
AID - 27_94 [pii]
AID - 10.1264/jsme2.me11230 [doi]
PST - ppublish
SO  - Microbes Environ. 2012;27(1):94-8. doi: 10.1264/jsme2.me11230. Epub 2011 Nov 10.