PMID- 22079351
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20220321
IS  - 1879-0429 (Electronic)
IS  - 0958-1669 (Linking)
VI  - 23
IP  - 1
DP  - 2012 Feb
TI  - New trends in fluorescence in situ hybridization for identification and 
      functional analyses of microbes.
PG  - 96-102
LID - 10.1016/j.copbio.2011.10.010 [doi]
AB  - Fluorescence in situ hybridization (FISH) has become an indispensable tool for 
      rapid and direct single-cell identification of microbes by detecting signature 
      regions in their rRNA molecules. Recent advances in this field include new 
      web-based tools for assisting probe design and optimization of experimental 
      conditions, easy-to-implement signal amplification strategies, innovative 
      multiplexing approaches, and the combination of FISH with transmission electron 
      microscopy or extracellular staining techniques. Further emerging developments 
      focus on sorting FISH-identified cells for subsequent single-cell genomics and on 
      the direct detection of specific genes within single microbial cells by advanced 
      FISH techniques employing various strategies for massive signal amplification.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Wagner, Michael
AU  - Wagner M
AD  - Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090 
      Vienna, Austria. wagner@microbial-ecology.net
FAU - Haider, Susanne
AU  - Haider S
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20111111
PL  - England
TA  - Curr Opin Biotechnol
JT  - Current opinion in biotechnology
JID - 9100492
RN  - 0 (DNA Primers)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA Probes)
SB  - IM
MH  - Archaea/genetics
MH  - Bacteria/genetics
MH  - DNA Primers/genetics
MH  - In Situ Hybridization, Fluorescence/*methods/trends
MH  - Oligonucleotide Probes/genetics
MH  - RNA Probes/genetics
EDAT- 2011/11/15 06:00
MHDA- 2012/10/16 06:00
CRDT- 2011/11/15 06:00
PHST- 2011/10/03 00:00 [received]
PHST- 2011/10/22 00:00 [accepted]
PHST- 2011/11/15 06:00 [entrez]
PHST- 2011/11/15 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
AID - S0958-1669(11)00706-3 [pii]
AID - 10.1016/j.copbio.2011.10.010 [doi]
PST - ppublish
SO  - Curr Opin Biotechnol. 2012 Feb;23(1):96-102. doi: 10.1016/j.copbio.2011.10.010. 
      Epub 2011 Nov 11.