PMID- 22081504
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20201209
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 51
IP  - 3
DP  - 2012 Mar
TI  - FLI1 is a novel ETS transcription factor involved in gene fusions in prostate 
      cancer.
PG  - 240-9
LID - 10.1002/gcc.20948 [doi]
AB  - To characterize the pattern of ETS rearrangements and to uncover novel ETS fusion 
      genes, we analyzed 200 prostate carcinomas (PCa) with TaqMan low-density arrays 
      (TLDAs), followed by selective analyses with fluorescence in situ hybridization 
      (FISH), RT-PCR, and sequencing. Besides confirming the recurrent presence of ERG, 
      ETV1, ETV4, and ETV5 rearrangements, we here report FLI1 as the fifth ETS 
      transcription factor involved in fusion genes in prostate cancer. Outlier 
      expression of the FLI1 gene was detected by TLDAs in one PCa that showed relative 
      overexpression of FLI1 exons 4:5 as compared with FLI1 exons 2:3. A structural 
      rearrangement was found using FISH probes flanking the FLI1 gene and RT-PCR and 
      sequencing analyses showed fusion of SLC45A3 exon 1 with FLI1 exon 3. 
      Interestingly, we found four cases with two different ETS rearrangements in the 
      index tumor, thus revealing intratumor genetic heterogeneity. Correlation 
      analysis with clinico-pathological data showed association of ERG rearrangements 
      with locally advanced disease (pT3, P = 0.007) and MYC overexpression (P = 
      0.001), and association of ETV1 rearrangements with PTEN downregulation (P = 
      0.015). We report that FLI1 is a novel ETS transcription factor involved in gene 
      fusions in prostate cancer and that intratumor genetic heterogeneity of ETS 
      rearrangements can occasionally be found in index primary tumors.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Paulo, Paula
AU  - Paulo P
AD  - Department of Genetics, Portuguese Oncology Institute, Porto, Portugal.
FAU - Barros-Silva, Joao D
AU  - Barros-Silva JD
FAU - Ribeiro, Franclim R
AU  - Ribeiro FR
FAU - Ramalho-Carvalho, Joao
AU  - Ramalho-Carvalho J
FAU - Jeronimo, Carmen
AU  - Jeronimo C
FAU - Henrique, Rui
AU  - Henrique R
FAU - Lind, Guro E
AU  - Lind GE
FAU - Skotheim, Rolf I
AU  - Skotheim RI
FAU - Lothe, Ragnhild A
AU  - Lothe RA
FAU - Teixeira, Manuel R
AU  - Teixeira MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111112
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERG protein, human)
RN  - 0 (ETV1 protein, human)
RN  - 0 (FLI1 protein, human)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (SLC45a3 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Regulator ERG)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Aged
MH  - Base Sequence
MH  - DNA-Binding Proteins/genetics
MH  - Exons
MH  - Gene Rearrangement
MH  - Genetic Heterogeneity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Membrane Transport Proteins/*genetics
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Monosaccharide Transport Proteins
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Oncogene Fusion
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Proto-Oncogene Protein c-fli-1/*genetics
MH  - Trans-Activators/genetics
MH  - Transcription Factors/genetics
MH  - Transcriptional Regulator ERG
EDAT- 2011/11/15 06:00
MHDA- 2012/09/05 06:00
CRDT- 2011/11/15 06:00
PHST- 2011/07/17 00:00 [received]
PHST- 2011/10/10 00:00 [accepted]
PHST- 2011/11/15 06:00 [entrez]
PHST- 2011/11/15 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - 10.1002/gcc.20948 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2012 Mar;51(3):240-9. doi: 10.1002/gcc.20948. Epub 2011 
      Nov 12.