PMID- 22081580
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 78
IP  - 2
DP  - 2012 Jan
TI  - Acinetobacter insertion sequence ISAba11 belongs to a novel family that encodes 
      transposases with a signature HHEK motif.
PG  - 471-80
LID - 10.1128/AEM.05663-11 [doi]
AB  - Experimental and in silico PCR analysis targeting ISAba11 and TnAbaR islands in 
      196 epidemiologically unrelated Acinetobacter strains representative of >/=19 
      species were performed. The first two Acinetobacter baumannii ISAba11 elements 
      identified had been found to map to the same site on TnAbaR transposons. However, 
      no further evidence of physical linkage between the two elements was 
      demonstrated. Indeed, examination of 25 definite or putative insertion sites 
      suggested limited sequence specificity. Importantly, an aacC1-tagged version of 
      ISAba11 was shown to actively transpose in A. baumannii. Similarity searches 
      identified nine iso-ISAba11 elements in Acinetobacter and one in Enhydrobacter 
      and single representatives of four distant homologs in bacteria belonging to the 
      phyla "Cyanobacteria" and Proteobacteria. Phylogenetic, sequence, and structural 
      analyses of ISAba11 and/or its associated transposase (Tnp(ISAba11)) suggested 
      that these elements be assigned to a new family. All five homologs encode 
      transposases with a shared extended signature comprising 16 invariant residues 
      within the N2, N3, and C1 regions, four of which constituted the cardinal ISAba11 
      family HHEK motif that is substituted for the YREK DNA binding motif conserved in 
      the IS4 family. Additionally, ISAba11 family members were associated with either 
      no flanking direct repeat (DR) or an ISAba11-typical 5-bp DR and possessed 
      variable-length terminal inverted repeats that exhibited extensive intrafamily 
      sequence identity. Given the limited pairwise identity among Tnp(ISAba11) 
      homologs and the observed restricted distribution of ISAba11, we propose that 
      substantial gaps persist in the evolutionary record of ISAba11 and that this 
      element represents a recent though potentially highly significant entrant into 
      the A. baumannii gene pool.
FAU - Rieck, Barbara
AU  - Rieck B
AD  - Department of Infection, Immunity and Inflammation, University of Leicester, 
      Leicester, United Kingdom.
FAU - Tourigny, David S
AU  - Tourigny DS
FAU - Crosatti, Marialuisa
AU  - Crosatti M
FAU - Schmid, Ralf
AU  - Schmid R
FAU - Kochar, Mandira
AU  - Kochar M
FAU - Harrison, Ewan M
AU  - Harrison EM
FAU - Ou, Hong-Yu
AU  - Ou HY
FAU - Turton, Jane F
AU  - Turton JF
FAU - Rajakumar, Kumar
AU  - Rajakumar K
LA  - eng
SI  - GENBANK/JN819186
SI  - GENBANK/JN819187
SI  - GENBANK/JN819188
SI  - GENBANK/JN819189
SI  - GENBANK/JN819190
SI  - GENBANK/JN819191
SI  - GENBANK/JN819192
SI  - GENBANK/JN819193
SI  - GENBANK/JN819194
SI  - GENBANK/JN819195
SI  - GENBANK/JN819196
SI  - GENBANK/JN819197
SI  - GENBANK/JN819198
SI  - GENBANK/JN819199
SI  - GENBANK/JN819200
SI  - GENBANK/JN819201
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111111
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - EC 2.7.7.- (Transposases)
SB  - IM
MH  - Acinetobacter/*enzymology/*genetics
MH  - *Amino Acid Motifs
MH  - Binding Sites
MH  - Cluster Analysis
MH  - *DNA Transposable Elements
MH  - DNA, Bacterial/chemistry/genetics
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Transposases/classification/*genetics
PMC - PMC3255748
EDAT- 2011/11/15 06:00
MHDA- 2012/04/20 06:00
PMCR- 2012/07/01
CRDT- 2011/11/15 06:00
PHST- 2011/11/15 06:00 [entrez]
PHST- 2011/11/15 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - AEM.05663-11 [pii]
AID - 5663-11 [pii]
AID - 10.1128/AEM.05663-11 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2012 Jan;78(2):471-80. doi: 10.1128/AEM.05663-11. Epub 
      2011 Nov 11.