PMID- 22084943
OWN - NLM
STAT- MEDLINE
DCOM- 20120823
LR  - 20120612
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 52
IP  - 6
DP  - 2012 Jun
TI  - Recovery, survival, and function of transfused platelets and detection of 
      platelet engraftment after allogeneic stem cell transplantation.
PG  - 1321-32
LID - 10.1111/j.1537-2995.2011.03442.x [doi]
AB  - BACKGROUND: Recovery and survival of transfused platelets (PLTs) are usually 
      assessed by radioisotope labeling methods for evaluation of transfusion efficacy 
      and new progress in the processing of PLT concentrates. Alternative, 
      nonradioactive methods are warranted. STUDY DESIGN AND METHODS: A multicolor flow 
      cytometry method was developed for simultaneous studies of recovery, survival, 
      and function of transfused PLTs. Eight consecutive patients undergoing allogeneic 
      stem cell transplantation (TX) were transfused with apheresis PLTs of nonself 
      human leukocyte antigen (HLA) Class I types, and HLA Class I discrepancy between 
      donor and recipient was used to identify transfused PLTs. Hematologic status and 
      HLA Class I surface expression were analyzed immediately before transfusion, 1 
      and 6 hours after transfusion, and daily during the subsequent week. PLT 
      activation was assessed by surface expression of CD63, CD62P, or CD42a, before 
      and after stimulation with thrombin receptor agonist peptide. RESULTS: PLT 
      recovery was 43, 41, and 31% for fresh (5-72 hr old) and 30, 27, and 17% for 
      stored (73-148 hr old) PLTs, after 1, 6, and 15 to 28 hours, respectively. 
      Survival of fresh versus stored PLTs were 160 and 105 hours, respectively. 
      Spontaneous PLT activation and residual activation potential were almost equal 
      for fresh and stored PLTs. PLT engraftment was detected between Day 7 and Day 9, 
      which was significantly earlier than first sign of neutrophil engraftment (Days 
      11-19; p=0.01). CONCLUSION: Flow cytometry is an attractive alternative to 
      radiolabeling of PLTs for simultaneous studies of survival, recovery, and 
      function of transfused PLTs and early detection of PLT engraftment after 
      allogeneic stem cell TX.
CI  - (c) 2011 American Association of Blood Banks.
FAU - Vetlesen, Annette
AU  - Vetlesen A
AD  - Department of Immunology and Transfusion Medicine, Oslo University Hospital HF, 
      Ullevaal, Norway. annette.vetlesen@medisin.uio.no
FAU - Holme, Pal Andre
AU  - Holme PA
FAU - Lyberg, Torstein
AU  - Lyberg T
FAU - Kjeldsen-Kragh, Jens
AU  - Kjeldsen-Kragh J
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
DEP - 20111116
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
SB  - IM
MH  - Adult
MH  - Blood Platelets/*cytology/*physiology
MH  - Blood Specimen Collection/methods
MH  - Cell Survival/physiology
MH  - Female
MH  - Graft Survival/*physiology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Platelet Count
MH  - *Platelet Transfusion/methods
MH  - Plateletpheresis/methods
MH  - Recovery of Function/physiology
MH  - Time Factors
MH  - Transplantation, Homologous
MH  - Young Adult
EDAT- 2011/11/17 06:00
MHDA- 2012/08/24 06:00
CRDT- 2011/11/17 06:00
PHST- 2011/11/17 06:00 [entrez]
PHST- 2011/11/17 06:00 [pubmed]
PHST- 2012/08/24 06:00 [medline]
AID - 10.1111/j.1537-2995.2011.03442.x [doi]
PST - ppublish
SO  - Transfusion. 2012 Jun;52(6):1321-32. doi: 10.1111/j.1537-2995.2011.03442.x. Epub 
      2011 Nov 16.