PMID- 22086396
OWN - NLM
STAT- MEDLINE
DCOM- 20120420
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 402
IP  - 3
DP  - 2012 Jan
TI  - Gold nanoparticle fluorescent molecular beacon for low-resolution DQ2 gene HLA 
      typing.
PG  - 1001-9
LID - 10.1007/s00216-011-5493-2 [doi]
AB  - Coeliac disease is an inflammation of the small intestine triggered by gluten 
      ingestion. We present a fluorescent genosensor, exploiting 
      molecular-beacon-functionalized gold nanoparticles, for the identification of 
      human leukocyte antigen (HLA) DQ2 gene, a key genetic factor in coeliac disease. 
      Optimization of sensor performance was achieved by tuning the composition of the 
      oligonucleotide monolayer immobilized on the gold nanoparticle and the molecular 
      beacon design. Co-immobilization of the molecular beacon with a spacing 
      oligonucleotide (thiolated ten-thymine oligonucleotide) in the presence of 
      ten-adenine oligonucleotides resulted in a significant increase of the sensor 
      response owing to improved spacing of the molecular beacons and extension of the 
      distance from the nanoparticle surface, which renders them more available for 
      recognition. Further increase in the response (approximately 40%) was shown to be 
      achievable when the recognition sequence of the molecular beacon was incorporated 
      in the stem. Improvement of the specificity of the molecular beacons was also 
      achieved by the incorporation within their recognition sequence of a one-base 
      mismatch. Finally, gold nanoparticles functionalized with two molecular beacons 
      targeting the DQA1*05* and DQB1*02* alleles allowed the low-resolution typing of 
      the DQ2 gene at the nanomolar level.
FAU - Beni, Valerio
AU  - Beni V
AD  - Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Quimica, 
      Universitat Rovira i Virgili, Av. Pasos Catalans, 26, 43007 Tarragona, Spain. 
      valerio.beni@urv.cat
FAU - Zewdu, Taye
AU  - Zewdu T
FAU - Joda, Hamdi
AU  - Joda H
FAU - Katakis, Ioanis
AU  - Katakis I
FAU - O'Sullivan, Ciara K
AU  - O'Sullivan CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111116
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Fluorescent Dyes)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ2 antigen)
RN  - 0 (Oligonucleotides)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Base Sequence
MH  - Calibration
MH  - Celiac Disease/genetics
MH  - Fluorescent Dyes/*chemistry
MH  - Genotype
MH  - Gold/*chemistry
MH  - HLA-DQ Antigens/*genetics
MH  - Humans
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Oligonucleotides/*chemistry
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/methods
EDAT- 2011/11/17 06:00
MHDA- 2012/04/21 06:00
CRDT- 2011/11/17 06:00
PHST- 2011/08/05 00:00 [received]
PHST- 2011/10/10 00:00 [accepted]
PHST- 2011/10/05 00:00 [revised]
PHST- 2011/11/17 06:00 [entrez]
PHST- 2011/11/17 06:00 [pubmed]
PHST- 2012/04/21 06:00 [medline]
AID - 10.1007/s00216-011-5493-2 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Jan;402(3):1001-9. doi: 10.1007/s00216-011-5493-2. Epub 
      2011 Nov 16.