PMID- 22087757
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20240507
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 4
DP  - 2011 Nov 16
TI  - Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray 
      analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis.
PG  - 25
LID - 10.1186/1755-8166-4-25 [doi]
AB  - BACKGROUND: Cytogenetic evaluation is a key component of the diagnosis and 
      prognosis of chronic lymphocytic leukemia (CLL). We performed 
      oligonucleotide-based comparative genomic hybridization microarray analysis on 34 
      samples with CLL and known abnormal karyotypes previously determined by 
      cytogenetics and/or fluorescence in situ hybridization (FISH). RESULTS: Using a 
      custom designed microarray that targets >1800 genes involved in hematologic 
      disease and other malignancies, we identified additional cryptic aberrations and 
      novel findings in 59% of cases. These included gains and losses of genes 
      associated with cell cycle regulation, apoptosis and susceptibility loci on 
      3p21.31, 5q35.2q35.3, 10q23.31q23.33, 11q22.3, and 22q11.23. CONCLUSIONS: Our 
      results show that microarray analysis will detect known aberrations, including 
      microscopic and cryptic alterations. In addition, novel genomic changes will be 
      uncovered that may become important prognostic predictors or treatment targets 
      for CLL in the future.
FAU - Kolquist, Kathryn A
AU  - Kolquist KA
AD  - Signature Genomic Laboratories, PerkinElmer Inc,, 2820 North Astor Street, 
      Spokane, WA, 99207, USA. lisa.shaffer@perkinelmer.com.
FAU - Schultz, Roger A
AU  - Schultz RA
FAU - Slovak, Marilyn L
AU  - Slovak ML
FAU - McDaniel, Lisa D
AU  - McDaniel LD
FAU - Brown, Theresa C
AU  - Brown TC
FAU - Tubbs, Raymond R
AU  - Tubbs RR
FAU - Cook, James R
AU  - Cook JR
FAU - Theil, Karl S
AU  - Theil KS
FAU - Cawich, Victoria
AU  - Cawich V
FAU - Valentin, Caitlin
AU  - Valentin C
FAU - Minier, Sara
AU  - Minier S
FAU - Neill, Nicholas J
AU  - Neill NJ
FAU - Byerly, Steve
AU  - Byerly S
FAU - Morton, S Annie
AU  - Morton SA
FAU - Sahoo, Trilochan
AU  - Sahoo T
FAU - Ballif, Blake C
AU  - Ballif BC
FAU - Shaffer, Lisa G
AU  - Shaffer LG
LA  - eng
PT  - Journal Article
DEP - 20111116
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC3253687
EDAT- 2011/11/18 06:00
MHDA- 2011/11/18 06:01
PMCR- 2011/11/16
CRDT- 2011/11/18 06:00
PHST- 2011/08/26 00:00 [received]
PHST- 2011/11/16 00:00 [accepted]
PHST- 2011/11/18 06:00 [entrez]
PHST- 2011/11/18 06:00 [pubmed]
PHST- 2011/11/18 06:01 [medline]
PHST- 2011/11/16 00:00 [pmc-release]
AID - 1755-8166-4-25 [pii]
AID - 10.1186/1755-8166-4-25 [doi]
PST - epublish
SO  - Mol Cytogenet. 2011 Nov 16;4:25. doi: 10.1186/1755-8166-4-25.