PMID- 2208792
OWN - NLM
STAT- MEDLINE
DCOM- 19901113
LR  - 20190510
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 82
IP  - 1
DP  - 1990 Oct
TI  - Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that 
      discriminate between human mantle zone and marginal zone B cells.
PG  - 181-7
AB  - Two new monoclonal antibodies (MoAbs), UCL3D3 and UCL4D12 were obtained following 
      immunization with follicular lymphoma (UCL3D3) or low-grade primary B cell 
      gastric lymphoma cells (UCL4D12). In normal splenic white pulp, tonsil and small 
      intestinal Peyer's patches, UCL4D12 recognizes marginal zone B cells and a 
      subpopulation of follicle centre cells, whereas mantle zone B cells are UCL4D12 
      negative. In contrast, UCL3D3 recognizes mantle zone B cells and follicular 
      dendritic cells, but not marginal zone B cells or follicle centre B cells. 
      Double-immunofluorescence studies showed that in the splenic white pulp, these 
      antibodies stain reciprocally. The majority of UCL3D3+ cells are sIgM+ and sIgD+ 
      whereas a higher proportion of UCL4D12+ cells express surface IgM (sIgM) but not 
      surface IgD (sIgD). Less than 10% of splenic B cells express both 3D3 and 4D12 
      antigens. None of the cell lines tested expressed either antigen. Functional 
      studies showed that both antigens play a role in B cell activation as the MoAbs 
      increase the mitogenic effect of Staphylococcus aureus Cowan I on tonsil B cells. 
      This effect was maximal at 72 h in culture. TPA activation was reduced, and no 
      effect was observed with anti-immunoglobulin (anti mu) or CDw40 (G28.5). UCL3D3 
      and UCL4D12 did not show any stimulatory effect on their own. Biochemical studies 
      show that both MoAbs recognize proteins of 80-90 kD under reducing conditions. 
      These two MoAbs appear to recognize new B cell surface antigens which may be 
      useful for identifying subpopulations of B cells.
FAU - Smith-Ravin, J
AU  - Smith-Ravin J
AD  - Imperial Cancer Research Fund-Human Tumour Immunology Group, Courtauld Institute, 
      London, England, U.K.
FAU - Spencer, J
AU  - Spencer J
FAU - Beverley, P C
AU  - Beverley PC
FAU - Isaacson, P G
AU  - Isaacson PG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/*immunology
MH  - Antigens, Surface/immunology
MH  - B-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Lymph Nodes/cytology/immunology
MH  - Lymphocyte Subsets/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Palatine Tonsil/cytology/immunology
MH  - Spleen/cytology/immunology
PMC - PMC1535176
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
PMCR- 1991/10/01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.1990.tb05424.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1990 Oct;82(1):181-7. doi: 10.1111/j.1365-2249.1990.tb05424.x.