PMID- 22088456
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20111117
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 124
IP  - 13
DP  - 2011 Jul 5
TI  - Role of tumor necrosis factor-alpha in the pathogenesis of atrial fibrillation.
PG  - 1976-82
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic proinflammatory 
      cytokine and contributes to many kinds of cardiovascular diseases via its 
      receptors (TNFR1/TNFR2). We hypothesize that TNF-alpha plays a role in the 
      pathogenesis of chronic atrial fibrillation (AF). METHODS: Sixty-seven 
      consecutive patients who were scheduled to have cardiac surgery were enrolled 
      into the study. Thirty-one patients with rheumatic heart disease (RHD) and AF 
      were enrolled as study group (AF group). The sinus rhythm (SR) control groups 
      consisted of 20 patients with RHD and 16 patients with coronary artery disease 
      (CAD). Peripheral blood sample was collected before the operation. About 5 mm(3) 
      left atrial tissue was disserted during the operation and was separated into 
      three parts for Western blotting, real time polymerase chain reaction (RT-PCR) 
      and immunohistochemistry (IHC) analysis. RESULTS: Compared with the controls (RHD 
      SR and CAD SR), the levels of TNF-alpha ((14.40 +/- 5.45) pg/ml vs. (4.20 +/- 3.19) pg/ml 
      vs. (2.68 +/- 2.20) pg/ml, P = 0.000) and its soluble receptor 1 (sTNFR1) ((1623.9 
      +/- 558.6) pg/ml vs. (1222.3 +/- 175.6) pg/ml vs. (1387.5 +/- 362.2) pg/ml, P = 0.001) 
      in plasma were higher in patients with AF. TNF-alpha level had positive correlation 
      with the left atrial diameter (LAD) (r = 0.642, P = 0.000). Western blotting 
      analysis showed that the protein levels of TNF-alpha (0.618 +/- 0.236 vs. 0.234 +/- 0.178 
      vs. 0.180 +/- 0.103, P = 0.000) were higher in patients with AF. The RT-PCR 
      analysis results demonstrated that the mRNA expression of TNF-alpha (0.103 +/- 0.047 
      vs. 0.031 +/- 0.027 vs. 0.023 +/- 0.018, P = 0.000) increased in patients with AF. 
      IHC analysis displayed that, comparing to the SR, the expression of TNF-alpha (0.125 
      +/- 0.025 vs. 0.080 +/- 0.027 vs. 0.070 +/- 0.023, P = 0.000) increased in the AF 
      group. The protein level and mRNA expression of TNF-alpha also had positive 
      correlation with left atrium diameter (LAD) (r = 0.415, P = 0.000 and r = 0.499, 
      P = 0.000). CONCLUSIONS: The results revealed that TNF-alpha elevated in the plasma 
      and left atrial tissue and had positive correlation with LAD in patients of 
      chronic AF. TNF-alpha might involve in the pathogenesis of chronic AF.
FAU - Deng, Hai
AU  - Deng H
AD  - Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, 
      China.
FAU - Xue, Yu-mei
AU  - Xue YM
FAU - Zhan, Xian-zhang
AU  - Zhan XZ
FAU - Liao, Hong-tao
AU  - Liao HT
FAU - Guo, Hui-ming
AU  - Guo HM
FAU - Wu, Shu-lin
AU  - Wu SL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Atrial Fibrillation/*blood/*metabolism
MH  - Blotting, Western
MH  - Female
MH  - Heart Atria/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Real-Time Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*blood/genetics/*metabolism
EDAT- 2011/11/18 06:00
MHDA- 2012/05/05 06:00
CRDT- 2011/11/18 06:00
PHST- 2011/11/18 06:00 [entrez]
PHST- 2011/11/18 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2011 Jul 5;124(13):1976-82.