PMID- 22088600
OWN - NLM
STAT- MEDLINE
DCOM- 20120703
LR  - 20111226
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 57
IP  - 1
DP  - 2012 Jan
TI  - The design and recombinant protein expression of a consensus porcine interferon: 
      CoPoIFN-alpha.
PG  - 37-45
LID - 10.1016/j.cyto.2011.10.011 [doi]
AB  - CoPoIFN-alpha is a recombinant non-naturally occurring porcine interferon-alpha (IFN-alpha). 
      It was designed by scanning 17 porcine IFN-alpha nonallelic subtypes and assigning 
      the most frequently occurring amino acid in each position. We used a porcine 
      IFN-alpha (PoIFN-alpha) derived from domestic pig as a control. Both porcine IFN-alpha genes 
      were introduced into yeast expression vector PpICZalpha-A and expressed in Pichia 
      pastoris. The antiviral unit of these two IFN-alphas were assayed in MDBK, PK-15 and 
      MARC-145 cells against vesicular stomatitis virus (VSV), and their inhibitory 
      abilities on pseudorabies virus (PRV) and porcine reproductive and respiratory 
      syndrome virus (PRRSV) replication were also examined, respectively. We found the 
      antiviral activity (units/mg) of CoPoIFN-alpha was 46.4, 63.6 and 53.5-fold higher 
      than that of PoIFN-alpha for VSV inhibition in MDBK, PK-15 and MARC-145 cells, 
      4.8-fold higher for PRV inhibition in PK-15 cells, and 5-fold higher for PRRSV 
      inhibition in MARC-145 cells. Our results also showed that the PRV and 
      PRRSV-specific cytopathic effect (CPE) could be inhibited in the cells pretreated 
      with CoPoIFN-alpha and PoIFN-alpha, and the virus titers in the cells pretreated with 
      CoPoIFN-alpha were lower than those cells pretreated with PoIFN-alpha by 10-20-fold. The 
      antiproliferative activity of CoPoIFN-alpha was significantly higher than that of 
      PoIFN-alpha on a molar basis. The mRNA level of Mx1 and OAS1 genes in PK-15 cells 
      induced by CoPoIFN-alpha were enhanced about 4.6-fold and 3.2-fold compared to that 
      induced by PoIFN-alpha. Based on a homology model of CoPoIFN-alpha and IFNAR2, all of the 
      different residues between native PoIFN-alpha and CoPoIFN-alpha were not involved in 
      IFNAR1 binding site, and there is no direct interaction between these residues 
      and IFNAR2, either. We speculate that the higher activity of CoPoIFN-alpha was likely 
      due to the electrostatic potential introduced by residue Arg156 around the 
      binding site or a structural perturbation caused by these different residues. 
      This may enhance the overall binding affinity of CoPoIIFN-alpha and the receptors. 
      Thus, CoPoIFN-alpha may have the potential to be used in therapy of porcine diseases.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Huang, Li
AU  - Huang L
AD  - Key Laboratory of Animal Disease Diagnostic & Immunology, Nanjing Agricultural 
      University, Nanjing, Jiangsu 210095, China.
FAU - Cao, Rui-bing
AU  - Cao RB
FAU - Wang, Ning
AU  - Wang N
FAU - Liu, Ke
AU  - Liu K
FAU - Wei, Jian-chao
AU  - Wei JC
FAU - Isahg, Hassan
AU  - Isahg H
FAU - Song, Li-jie
AU  - Song LJ
FAU - Zuo, Wei-yong
AU  - Zuo WY
FAU - Zhou, Bin
AU  - Zhou B
FAU - Wang, Wei-wu
AU  - Wang WW
FAU - Mao, Xiang
AU  - Mao X
FAU - Chen, Pu-yan
AU  - Chen PY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111115
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antiviral Agents)
RN  - 0 (Interferon-alpha)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antiviral Agents/pharmacology
MH  - Blotting, Western
MH  - Cattle
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Circular Dichroism
MH  - *Consensus Sequence
MH  - Cytopathogenic Effect, Viral/drug effects
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Herpesvirus 1, Suid/drug effects
MH  - Interferon-alpha/*biosynthesis/chemistry/*genetics/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Pichia
MH  - Plasmids/genetics
MH  - Porcine respiratory and reproductive syndrome virus/drug effects
MH  - Protein Structure, Secondary
MH  - Recombinant Proteins/chemistry/genetics/*metabolism
MH  - Sequence Alignment
MH  - Structural Homology, Protein
MH  - Sus scrofa
MH  - Up-Regulation/drug effects
MH  - Vesiculovirus/drug effects
MH  - Virus Replication/drug effects
EDAT- 2011/11/18 06:00
MHDA- 2012/07/04 06:00
CRDT- 2011/11/18 06:00
PHST- 2010/12/18 00:00 [received]
PHST- 2011/09/01 00:00 [revised]
PHST- 2011/10/20 00:00 [accepted]
PHST- 2011/11/18 06:00 [entrez]
PHST- 2011/11/18 06:00 [pubmed]
PHST- 2012/07/04 06:00 [medline]
AID - S1043-4666(11)00798-8 [pii]
AID - 10.1016/j.cyto.2011.10.011 [doi]
PST - ppublish
SO  - Cytokine. 2012 Jan;57(1):37-45. doi: 10.1016/j.cyto.2011.10.011. Epub 2011 Nov 
      15.