PMID- 22090289
OWN - NLM
STAT- MEDLINE
DCOM- 20120809
LR  - 20191210
IS  - 0374-9096 (Print)
IS  - 0374-9096 (Linking)
VI  - 45
IP  - 4
DP  - 2011 Oct
TI  - [Evaluation of rapid genotype assay for the identification of gram-positive cocci 
      from blood cultures and detection of mecA and van genes].
PG  - 592-601
AB  - Rapid and accurate identification of bacterial pathogens grown in blood cultures 
      of patients with sepsis is crucial for prompt initiation of appropriate therapy 
      in order to decrease related morbidity and mortality rates. Although current 
      automated blood culture systems led to a significant improvement in bacterial 
      detection time, more rapid identification systems are still needed to optimise 
      the establishment of treatment. Novel genotype technology which is developed for 
      the rapid diagnosis of sepsis, is a molecular genetic assay based on DNA 
      multiplex amplification with biotinylated primers followed by hybridization to 
      membrane bound probes. The aim of this study was to evaluate the performance of 
      "Genotype(R) BC gram-positive” test for the identification of gram-positive cocci 
      grown in blood cultures and rapid detection of mecA and van genes. This test uses 
      DNA.STRIP(R) technology which includes a panel of probes for identification of 17 
      gram-positive bacterial species and is able to determinate the methicillin and 
      vancomycin resistance mediating genes (mecA and vanA, vanB, vanC1, vanC2/C3) 
      simultaneously, in a single test run. A total of 55 positive blood cultures from 
      BACTECTM Plus/F (Becton Dickinson, USA) aerobic and pediatric blood culture vials 
      were included in the study. The isolates which exhibit gram-positive coccus 
      morphology by Gram staining were identified by Genotype (R) BC gram-positive test 
      (Hain Life Science, Germany). All of the samples were also identified with the 
      use of Phoenix PMIC/ID Panel (Becton Dickinson, USA) and antibiotic 
      susceptibilities were determined. Of the 55 blood culture isolates, 17 were 
      identified as Staphylococcus epidermidis [all were methicillin-resistant (MR)], 9 
      were S.aureus (one was MR), 18 were S.hominis (10 were MR), 4 were E.faecalis, 3 
      were E. faecium (one was vanconycin-resistant), 2 were S.saprophyticus (one was 
      MR), 1 was S.warneri and 1 was S.haemolyticus, by Phoenix automated system. 
      Genotype(R) BC gram-positive test results revealed consistency with Phoenix system 
      regarding bacterial identification in 46 (83.6%) of the samples. The two bacteria 
      identified as S.saprophyticus by the Phoenix system could not be identified by 
      the Genotype(R) BC test since this species were not included in the identification 
      panel of the system, however, mecA gene were detected in these two samples by 
      Genotype(R) BC test. Genotype(R) BC test detected mecA gene in five samples which 
      were not detected as methicillin resistant by the Phoenix system. Besides 
      polymicrobial growth was determined in five samples by Genotype (R) BC test, but 
      not by the automated system. One E.faecium isolate with vanA gene was correctly 
      identified by Genotype(R) BC test. In conclusion, Genotype(R) BC gram-positive test 
      is a fast and reliable test for the identification of the most important 
      gram-positive pathogens and mecA and van genes directly from positive blood 
      culture bottles. This test was also found superior than the automated Phoenix 
      system regarding the detection of polymicrobial growth. These data indicated 
      that, routine use of DNA strip technology-based assay would be useful for 
      clinical diagnosis in patients with sepsis.
FAU - Gulhan, Baris
AU  - Gulhan B
AD  - Tatvan State Hospital, Bitlis, Turkey. barisgulhan@gmail.com
FAU - Atmaca, Selahattin
AU  - Atmaca S
FAU - Ozekinci, Tuncer
AU  - Ozekinci T
FAU - Suay, Adnan
AU  - Suay A
LA  - tur
PT  - Evaluation Study
PT  - Journal Article
TT  - Kan Kulturlerinde Ureyen Gram-Pozitif Koklarin Tanimlanmasi ve mecA ve van 
      Genlerinin Saptanmasinda Hizli Genotip Testinin Degerlendirilmesi.
PL  - Turkey
TA  - Mikrobiyol Bul
JT  - Mikrobiyoloji bulteni
JID - 7503830
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Bacteremia/diagnosis/*microbiology
MH  - Bacterial Proteins/*genetics
MH  - Genotyping Techniques/*standards
MH  - Gram-Positive Bacterial Infections/diagnosis/*microbiology
MH  - Gram-Positive Cocci/*classification/genetics/isolation & purification
MH  - Humans
MH  - Methicillin Resistance/genetics
MH  - Time Factors
MH  - Vancomycin Resistance/genetics
EDAT- 2011/11/18 06:00
MHDA- 2012/08/10 06:00
CRDT- 2011/11/18 06:00
PHST- 2011/11/18 06:00 [entrez]
PHST- 2011/11/18 06:00 [pubmed]
PHST- 2012/08/10 06:00 [medline]
PST - ppublish
SO  - Mikrobiyol Bul. 2011 Oct;45(4):592-601.