PMID- 22093690
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20200319
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Linking)
VI  - 87
IP  - 2-3
DP  - 2012 Feb 10
TI  - Phenotypic characteristics of hybrid cells generated by transferring neuronal 
      nuclei into bone marrow stromal cell cytoplasts.
PG  - 303-11
LID - 10.1016/j.brainresbull.2011.11.001 [doi]
AB  - Bone marrow stromal cells (BMSCs) are promising donor cells for transplantation 
      therapies for a variety of diseases. However, there still lack efficient ways to 
      induce directional differentiation of BMSCs to promote their practical use in 
      transplantation therapy. In this study, we constructed hybrid cells by 
      transferring neuronal nuclei into BMSC cytoplasts and investigated the 
      proliferative capacity and phenotypic characteristics of the hybrid cells. The 
      neuronal nuclei were labeled with Hoechst 33342 before the transfer process, and 
      the cell membrane antigen CD71 was used as a marker of BMSC cytoplasts. The BMSC 
      cytoplasts and neuronal karyoplasts were separated by Ficoll density gradient 
      ultracentrifugation. The hybrid cells were generated by the polyethylene 
      glycol-mediated fusion of BMSC cytoplasts with neuronal karyoplasts. The hybrid 
      cells exhibited Hoechst 33342 staining in their nuclei and CD71 staining on their 
      cytomembranes, which confirmed the success of cell fusion. The hybrid cells were 
      positive for BrdU immunostaining. Viability analysis of the cultured hybrid cells 
      by the MTT assay demonstrated their proliferative ability. Immunocytochemical 
      staining revealed the expression of the neuron-specific markers NeuN and MAP2 in 
      the third passage hybrid cells, which indicated their neuronal phenotypic 
      characteristics. The results demonstrated that the hybrid cells produced by 
      fusing neuronal karyoplasts with BMSC cytoplasts had proliferative capability and 
      expressed the neuron-specific markers. Further study is required to investigate 
      the phenotype of the hybrid cells both structurally and functionally.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Zhou, Zhujuan
AU  - Zhou Z
AD  - Department of Neurology, Xinqiao Hospital, Third Military Medical University, 
      Chongqing 400037, China.
FAU - Xu, Yan
AU  - Xu Y
FAU - Zhong, Qi
AU  - Zhong Q
FAU - Zheng, Jian
AU  - Zheng J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111111
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Antigens, CD)
RN  - 0 (CD71 antigen)
RN  - 0 (MAP2 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Count
MH  - Cell Differentiation/physiology
MH  - Cell Fusion/methods
MH  - Cell Nucleus/*physiology/ultrastructure
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Embryo, Mammalian
MH  - Hybrid Cells/*physiology
MH  - Microscopy, Electron, Transmission
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/*cytology
MH  - *Phenotype
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Transferrin/metabolism
MH  - Stromal Cells/*physiology
MH  - Time Factors
EDAT- 2011/11/19 06:00
MHDA- 2012/08/02 06:00
CRDT- 2011/11/19 06:00
PHST- 2010/06/01 00:00 [received]
PHST- 2011/10/09 00:00 [revised]
PHST- 2011/11/02 00:00 [accepted]
PHST- 2011/11/19 06:00 [entrez]
PHST- 2011/11/19 06:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
AID - S0361-9230(11)00327-3 [pii]
AID - 10.1016/j.brainresbull.2011.11.001 [doi]
PST - ppublish
SO  - Brain Res Bull. 2012 Feb 10;87(2-3):303-11. doi: 
      10.1016/j.brainresbull.2011.11.001. Epub 2011 Nov 11.