PMID- 22095101
OWN - NLM
STAT- MEDLINE
DCOM- 20120817
LR  - 20220311
IS  - 1432-1440 (Electronic)
IS  - 0946-2716 (Print)
IS  - 0946-2716 (Linking)
VI  - 90
IP  - 4
DP  - 2012 Apr
TI  - Comparative kinome analysis to identify putative colon tumor biomarkers.
PG  - 447-56
LID - 10.1007/s00109-011-0831-6 [doi]
AB  - Kinase domains are the type of protein domain most commonly found in genes 
      associated with tumorigenesis. Because of this, the human kinome (the protein 
      kinase component of the genome) represents a promising source of cancer 
      biomarkers and potential targets for novel anti-cancer therapies. Alterations in 
      the human colon kinome during the progression from normal colon (NC) through 
      adenoma (AD) to adenocarcinoma (AC) were investigated using integrated 
      transcriptomic and proteomic datasets. Two hundred thirty kinase genes and 42 
      kinase proteins showed differential expression patterns (fold change >/= 1.5) in at 
      least one tissue pair-wise comparison (AD vs. NC, AC vs. NC, and/or AC vs. AD). 
      Kinases that exhibited similar trends in expression at both the mRNA and protein 
      levels were further analyzed in individual samples of NC (n = 20), AD (n = 39), 
      and AC (n = 24) by quantitative reverse transcriptase PCR. Individual samples of 
      NC and tumor tissue were distinguishable based on the mRNA levels of a set of 20 
      kinases. Altered expression of several of these kinases, including chaperone 
      activity of bc1 complex-like (CABC1) kinase, bromodomain adjacent to zinc finger 
      domain protein 1B (BAZ1B) kinase, calcium/calmodulin-dependent protein kinase 
      type II subunit delta (CAMK2D), serine/threonine-protein kinase 24 (STK24), 
      vaccinia-related kinase 3 (VRK3), and TAO kinase 3 (TAOK3), has not been 
      previously reported in tumor tissue. These findings may have diagnostic potential 
      and may lead to the development of novel targeted therapeutic interventions for 
      colorectal cancer.
FAU - Hennig, Ewa E
AU  - Hennig EE
AD  - Department of Gastroenterology and Hepatology, Medical Center for Postgraduate 
      Education, Warsaw, Poland.
FAU - Mikula, Michal
AU  - Mikula M
FAU - Rubel, Tymon
AU  - Rubel T
FAU - Dadlez, Michal
AU  - Dadlez M
FAU - Ostrowski, Jerzy
AU  - Ostrowski J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111118
PL  - Germany
TA  - J Mol Med (Berl)
JT  - Journal of molecular medicine (Berlin, Germany)
JID - 9504370
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Adenocarcinoma/diagnosis/enzymology/*genetics
MH  - Adenoma/diagnosis/enzymology/*genetics
MH  - Biomarkers, Tumor/analysis/genetics
MH  - Colonic Neoplasms/diagnosis/enzymology/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Protein Kinases/*genetics
MH  - Proteomics
MH  - RNA, Messenger/genetics
MH  - Transcriptome
PMC - PMC3307991
EDAT- 2011/11/19 06:00
MHDA- 2012/08/18 06:00
PMCR- 2011/11/18
CRDT- 2011/11/19 06:00
PHST- 2011/06/30 00:00 [received]
PHST- 2011/10/28 00:00 [accepted]
PHST- 2011/10/22 00:00 [revised]
PHST- 2011/11/19 06:00 [entrez]
PHST- 2011/11/19 06:00 [pubmed]
PHST- 2012/08/18 06:00 [medline]
PHST- 2011/11/18 00:00 [pmc-release]
AID - 831 [pii]
AID - 10.1007/s00109-011-0831-6 [doi]
PST - ppublish
SO  - J Mol Med (Berl). 2012 Apr;90(4):447-56. doi: 10.1007/s00109-011-0831-6. Epub 
      2011 Nov 18.