PMID- 22096343
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111123
LR  - 20211021
IS  - 1178-7031 (Print)
IS  - 1178-7031 (Electronic)
IS  - 1178-7031 (Linking)
VI  - 1
DP  - 2008
TI  - No evidence for activation of T(H)1 or T(H)17 pathways in unstimulated peripheral 
      blood mononuclear cells from children with beta-cell autoimmunity or T1D.
PG  - 11-7
AB  - INTRODUCTION: The balance between T(H)1, T(H)2, T(H)17, and regulatory T cells 
      has been suggested to be disturbed in type 1 diabetes (T1D). We investigated this 
      balance in peripheral blood mononuclear cells (PBMC) from children at risk of 
      developing T1D and children with T1D. METHODS: We studied PBMC expression levels 
      of markers related to T(H)1 (T-bet, IL-12Rbeta(1), IL-12Rbeta(2)), T(H)2 (GATA-3, 
      IL-4Ralpha), T(H)17 (IL-17A), and regulatory T cells (Foxp3, ICOS, and CTLA-4) with 
      real-time polymerase chain reaction from 17 children with T1D, 13 children with 
      beta-cell autoimmunity, 15 children with T1D risk-associated human leukocyte antigen 
      (HLA) haplotypes, and 24 healthy, control children. RESULTS: We observed 
      decreased expression levels of GATA-3 by PBMC of healthy children with 
      autoantibodies compared to healthy, control children (p = 0.014) or children with 
      HLA risk alleles (p = 0.032). Children with T1D demonstrated lower expression 
      levels of T-bet, IL-12Rbeta(1), and IL-4Ralpha both at diagnosis and 12 months later. 
      CONCLUSION: We found no indication of aberrant activation of T(H)1, T(H)17, or 
      Treg in peripheral blood from children with or without risk of T1D. The observed 
      immunological differences between children at risk of and with T1D should be 
      considered when immunopathogenesis of beta-cell destruction is studied.
FAU - Wallden, Jenny
AU  - Wallden J
AD  - Linkoping University, Faculty of Health Sciences, Department of Clinical and 
      Experimental Medicine, Division of Pediatrics and Diabetes Research Center, 
      Linkoping, Sweden.
FAU - Honkanen, Jarno
AU  - Honkanen J
FAU - Ilonen, Jorma
AU  - Ilonen J
FAU - Ludvigsson, Johnny
AU  - Ludvigsson J
FAU - Vaarala, Outi
AU  - Vaarala O
LA  - eng
PT  - Journal Article
DEP - 20080901
PL  - New Zealand
TA  - J Inflamm Res
JT  - Journal of inflammation research
JID - 101512684
PMC - PMC3218718
OTO - NOTNLM
OT  - mononuclear cells
OT  - transcription factor
OT  - type 1 diabetes
EDAT- 2008/01/01 00:00
MHDA- 2008/01/01 00:01
PMCR- 2008/09/01
CRDT- 2011/11/19 06:00
PHST- 2011/11/19 06:00 [entrez]
PHST- 2008/01/01 00:00 [pubmed]
PHST- 2008/01/01 00:01 [medline]
PHST- 2008/09/01 00:00 [pmc-release]
AID - jir_1_11 [pii]
AID - 10.2147/jir.s3547 [doi]
PST - ppublish
SO  - J Inflamm Res. 2008;1:11-7. doi: 10.2147/jir.s3547. Epub 2008 Sep 1.