PMID- 2209729
OWN - NLM
STAT- MEDLINE
DCOM- 19901105
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 190
IP  - 2
DP  - 1990 Oct
TI  - Tubular lysosomes and their drug reactivity in cultured resident macrophages and 
      in cell-free medium.
PG  - 283-9
AB  - Lysosomes were assessed in normal living resident mouse peritoneal macrophages, 
      using mainly phase-contrast microscopy (PCM), darkfield microscopy (DFM), and 
      fluorescence microscopy (FM) after terminal acridine orange (AO) staining; these 
      procedures avoided dyes during experimentation. After a few hours of culture a 
      variable proportion of the normal spherical lysosomes began to assemble in a 
      linear fashion. Fully formed tubular structures, with appearances generally 
      recognized as characteristic of tubular lysosomes (TL), could be seen by PCM or, 
      after labeling, by FM, at 2-5 days (best usually at 4-5 days). This peak was 
      followed by a reduction, and at 8-10 days most of the TL had disappeared, leaving 
      only spherical lysosomes. Renewal of the medium at this stage was followed by a 
      temporary reappearance of TL, suggesting that the medium was a major factor in 
      their initial development also. Formation of TL was enhanced by chloroquine (Cq), 
      though to a lesser degree than by phorbol ester (PMA); in contrast NH4Cl (like Cq 
      a weakly basic amine) caused their disassembly into spherical lysosomes. Manual 
      disruption of the monolayer macrophages enabled TL to be transferred to a 
      cell-free medium, in which they remained apparently stable for several hours. Two 
      known microtubule depolymerizers caused disassembly of TL in the intact cells, 
      reinforcing the idea that the TL are associated with the cytoplasmic microtubule 
      (MT) system; but these agents were inactive in vitro, suggesting that 
      disorganization of the system was responsible for this change.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Young, M R
AU  - Young MR
AD  - Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical 
      Research, London, United Kingdom.
FAU - Gordon, A H
AU  - Gordon AH
FAU - Hart, P D
AU  - Hart PD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Culture Media)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 886U3H6UFF (Chloroquine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SH1WY3R615 (Nocodazole)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Ammonium Chloride/*pharmacology
MH  - Animals
MH  - Cell-Free System
MH  - Cells, Cultured
MH  - Chloroquine/*pharmacology
MH  - Colchicine/pharmacology
MH  - Culture Media/pharmacology
MH  - Female
MH  - Lysosomes/*drug effects/metabolism/ultrastructure
MH  - Macrophages/*cytology/drug effects/ultrastructure
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Microtubules/drug effects/metabolism/ultrastructure
MH  - Nocodazole/pharmacology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1016/0014-4827(90)90198-j [doi]
PST - ppublish
SO  - Exp Cell Res. 1990 Oct;190(2):283-9. doi: 10.1016/0014-4827(90)90198-j.