PMID- 22097786
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161125
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 7
DP  - 2011 Jul
TI  - [Determination of five aflatoxins in Chinese patent medicines and medicinal herbs 
      by immunoaffinity extraction coupled with ultra-high performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 613-7
AB  - A method for the determination of five aflatoxins (B1 , B2, G1 , G2, M1 ) in 
      Chinese patent medicines and medicinal herbs by immunoaffinity extraction coupled 
      with ultra-high performance liquid chromatography-tandem mass spectrometry 
      (UHPLC-MS/MS) was developed. The samples were extracted with 80% (v/v) 
      methanol-water solution, followed by stepwise purification using an 
      immunoaffinity column. The target compounds were then eluted with methanol. The 
      extract was filtered then analyzed. With the gradient elution using a binary 
      mobile phase containing of 0.1% formic acid-5 mmol/L ammonium acetate solution 
      and methanol, the five aflatoxins were separated on an UHPLC BEH C18 column, 
      followed by positive electrospray ionization and multi-reaction monitoring (MRM) 
      provided by a triple-quadrupole tandem mass spectrometer. The limits of detection 
      for the standard solution of aflatoxins ranged from 0.05-0.3 microg/L. The linear 
      response was observed in the spiked concentration range of 0.5-100 microg/L with 
      the correlation coefficients higher than 0.99. The spiked recoveries were within 
      62.3%-82.4% at the spiked levels of 1.0 microg/kg and 5.0 microg/kg for all the 
      five aflatoxins with the relative standard deviations (RSDs) below 10% (n = 6). 
      The developed method is sensitive, accurate, and reproducible with the reasonable 
      recoveries, and can be applied to the determination of the 5 aflatoxins in the 
      Chinese traditional patent medicines, medicinal herbs as well as other similar 
      complex matrices.
FAU - Han, Shen
AU  - Han S
AD  - Technical Centre of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 
      100026, China.
FAU - Liu, Ying
AU  - Liu Y
FAU - Lu, Meiling
AU  - Lu M
FAU - Li, Jianzhong
AU  - Li J
FAU - Wang, Jinhua
AU  - Wang J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Aflatoxins)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 1DB78J7PUD (aflatoxin G1)
RN  - 7SKR7S646P (aflatoxin B2)
RN  - 9N2N2Y55MH (Aflatoxin B1)
SB  - IM
MH  - Aflatoxin B1/analysis
MH  - Aflatoxins/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Drug Contamination
MH  - Drugs, Chinese Herbal/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/11/22 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/11/22 06:00
PHST- 2011/11/22 06:00 [entrez]
PHST- 2011/11/22 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Jul;29(7):613-7.