PMID- 22098717
OWN - NLM
STAT- MEDLINE
DCOM- 20120308
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 32
DP  - 2011 Dec 15
TI  - Capillary electrophoretic determination of DNA damage markers: content of 
      8-hydroxy-2'-deoxyguanosine and 8-nitroguanine in urine.
PG  - 3818-22
LID - 10.1016/j.jchromb.2011.10.026 [doi]
AB  - A sensitive and low-cost analytical method has been developed to determine 
      8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-nitroguanine (8-NO(2)Gua) based on 
      capillary electrophoresis with amperometric detection (CE-AD) after solid phase 
      extraction (SPE). Under optimized condition, these two markers were well 
      separated from other components coexisting in urine, exhibiting a linear 
      calibration over the concentration range of 0.1-50.0 mug/mL with the detection 
      limits ranging from 0.02 to 0.06 mug/mL. The relative standard deviations (RSDs) 
      were in the range of 0.1-2.1% for peak area, 0.1-1.5% for migration time, 
      respectively. The average recovery and RSD were within the range of 100.0-108.0% 
      and 0.1-1.7%, respectively. It was found that the urinary contents of 8-OHdG and 
      8-NO(2)Gua in cancer patients were significantly higher than those in healthy 
      ones.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Li, Meng-Jie
AU  - Li MJ
AD  - Department of Chemistry, East China Normal University, Shanghai, China.
FAU - Zhang, Jun-Bo
AU  - Zhang JB
FAU - Li, Wen-Li
AU  - Li WL
FAU - Chu, Qing-Cui
AU  - Chu QC
FAU - Ye, Jian-Nong
AU  - Ye JN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111029
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (8-nitroguanine)
RN  - 0 (Biomarkers)
RN  - 5Z93L87A1R (Guanine)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adult
MH  - Aged
MH  - Biomarkers/*urine
MH  - *DNA Damage
MH  - Deoxyguanosine/*analogs & derivatives/urine
MH  - Electrophoresis, Capillary/*methods
MH  - Female
MH  - Guanine/*analogs & derivatives/urine
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/urine
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
EDAT- 2011/11/22 06:00
MHDA- 2012/03/09 06:00
CRDT- 2011/11/22 06:00
PHST- 2011/07/04 00:00 [received]
PHST- 2011/10/19 00:00 [revised]
PHST- 2011/10/23 00:00 [accepted]
PHST- 2011/11/22 06:00 [entrez]
PHST- 2011/11/22 06:00 [pubmed]
PHST- 2012/03/09 06:00 [medline]
AID - S1570-0232(11)00693-3 [pii]
AID - 10.1016/j.jchromb.2011.10.026 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Dec 15;879(32):3818-22. doi: 
      10.1016/j.jchromb.2011.10.026. Epub 2011 Oct 29.