PMID- 22100552
OWN - NLM
STAT- MEDLINE
DCOM- 20120308
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 32
DP  - 2011 Dec 15
TI  - A rapid and sensitive method for the analysis of brain monoamine 
      neurotransmitters using ultra-fast liquid chromatography coupled to 
      electrochemical detection.
PG  - 3871-8
LID - 10.1016/j.jchromb.2011.10.038 [doi]
AB  - Electrochemical detection is often used to detect catecholamines and indolamines 
      in brain samples that have been separated by conventional reverse-phase high 
      performance liquid chromatography (HPLC). This paper presents the transfer of an 
      existing chromatographic method for the determination of monoamines in brain 
      tissues using 5 mum granulometry HPLC columns to columns with a particle diameter 
      less than 3 mum. Several parameters (repeatability, linearity, accuracy, limit of 
      detection, and stability of samples) for this new ultrafast high performance 
      liquid chromatography (UHPLC) method were examined after optimization of the 
      analytical conditions. The separation of seven compounds, noradrenaline, dopamine 
      and three of its metabolites, dihydroxyphenylacetic acid, homovanillic acid, and 
      3-methoxytyramine, and serotonin and its metabolite, 5-hydroxyindole-3-acetic 
      acid was analyzed using this UHPLC-electrochemical detection method. The final 
      method, which was applied to brain tissue extracts from mice, rats, and cats, 
      decreased analysis time by a factor of 4 compared to HPLC, while guaranteeing 
      good analytical performance.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Parrot, Sandrine
AU  - Parrot S
AD  - INSERM U1028, Lyon Neuroscience Research Center, Ani-RA NeuroChem, Lyon, France. 
      sandrine.parrot@univ-lyon1.fr
FAU - Neuzeret, Pierre-Charles
AU  - Neuzeret PC
FAU - Denoroy, Luc
AU  - Denoroy L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111103
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Catecholamines)
RN  - 0 (Neurotransmitter Agents)
RN  - 102-32-9 (3,4-Dihydroxyphenylacetic Acid)
RN  - 333DO1RDJY (Serotonin)
RN  - X77S6GMS36 (Homovanillic Acid)
SB  - IM
MH  - 3,4-Dihydroxyphenylacetic Acid/analysis
MH  - Animals
MH  - *Brain Chemistry
MH  - Catecholamines/*analysis
MH  - Cats
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Stability
MH  - Homovanillic Acid/analysis
MH  - Limit of Detection
MH  - Mice
MH  - Neurotransmitter Agents/*analysis
MH  - Rats
MH  - Reproducibility of Results
MH  - Serotonin/*analysis
EDAT- 2011/11/22 06:00
MHDA- 2012/03/09 06:00
CRDT- 2011/11/22 06:00
PHST- 2011/09/23 00:00 [received]
PHST- 2011/10/27 00:00 [revised]
PHST- 2011/10/29 00:00 [accepted]
PHST- 2011/11/22 06:00 [entrez]
PHST- 2011/11/22 06:00 [pubmed]
PHST- 2012/03/09 06:00 [medline]
AID - S1570-0232(11)00714-8 [pii]
AID - 10.1016/j.jchromb.2011.10.038 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Dec 15;879(32):3871-8. doi: 
      10.1016/j.jchromb.2011.10.038. Epub 2011 Nov 3.