PMID- 22103739
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20160511
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Linking)
VI  - 59
IP  - 4
DP  - 2012 Aug
TI  - Analysis of the complete genome of Tembusu virus, a flavivirus isolated from 
      ducks in China.
PG  - 336-43
LID - 10.1111/j.1865-1682.2011.01275.x [doi]
AB  - During investigations into the outbreak of duck viral infection in 2010 in China, 
      with a severe drop in egg production, a flavivirus was isolated from the affected 
      ducks. It was characterized as a Tembusu virus (TMUV). In this study, we obtained 
      a complete genome sequence of Tembusu virus using RT-PCR and RACE techniques. 
      TMUV genome is a singled-stranded RNA, with 10,990 nucleotides in length, and 
      contains a single open reading frame (3410 amino acids) encoding 11 viral 
      proteins with 5'and 3'non-translated regions (NTRs) of 142 and 618 nt, 
      respectively. We characterized the open reading frame (ORF) with respect to gene 
      sizes, cleavage sites and potential glycosylation sites. The different genomic 
      regions of the virus were also compared with those of six other flaviviruses 
      including Japanese encephalitis virus, West Nile virus (WNV), dengue-2 virus, 
      yellow fever virus, tick-borne encephalitis virus (TBEV) and Bagaza virus. TMUV 
      demonstrated the highest similarity to Bagaza virus. The result of entire ORF 
      scanning shows that TMUV was close to Bagaza viruses in genetic relatedness. 
      These data demonstrate that TMUV is a unique virus among the mosquito-borne 
      flaviviruses and also provide a useful reference for a critically important study 
      to determine why TMUV is a serious pathogen for ducks.
CI  - (c) 2011 Blackwell Verlag GmbH.
FAU - Tang, Y
AU  - Tang Y
AD  - Zoology Institute, Agricultural University of Shan Dong Province, Shan Dong 
      Province, China.
FAU - Diao, Y
AU  - Diao Y
FAU - Gao, X
AU  - Gao X
FAU - Yu, C
AU  - Yu C
FAU - Chen, L
AU  - Chen L
FAU - Zhang, D
AU  - Zhang D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111122
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - China/epidemiology
MH  - Ducks/*virology
MH  - Flavivirus/*genetics
MH  - Flavivirus Infections/genetics/*veterinary
MH  - Gene Expression Regulation, Viral/physiology
MH  - Genome, Viral
MH  - Phylogeny
MH  - Poultry Diseases/epidemiology/*virology
MH  - RNA, Viral/genetics
MH  - Viral Proteins/genetics/metabolism
EDAT- 2011/11/23 06:00
MHDA- 2012/12/12 06:00
CRDT- 2011/11/23 06:00
PHST- 2011/11/23 06:00 [entrez]
PHST- 2011/11/23 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - 10.1111/j.1865-1682.2011.01275.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2012 Aug;59(4):336-43. doi: 
      10.1111/j.1865-1682.2011.01275.x. Epub 2011 Nov 22.